Adhya S, Echols H
J Bacteriol. 1966 Sep;92(3):601-8. doi: 10.1128/jb.92.3.601-608.1966.
Adhya, Sankar (University of Wisconsin, Madison), and Harrison Echols. Glucose effect and the galactose enzymes of Escherichia coli: correlation between glucose inhibition of induction and inducer transport. J. Bacteriol. 92:601-608. 1966.-The inhibitory effect of glucose on the induction of the enzymes required for galactose utilization ("glucose effect") was studied in Escherichia coli. Experiments on the uptake into the cell of labeled inducers (d-galactose-C(14) and d-fucose-H(3)) pointed to inhibition at the level of inducer transport as the possible primary mechanism of the glucose effect in the case of the gal enzymes. This interpretation was supported by the finding that a mutant constitutive for the lac enzymes was resistant to glucose inhibition of galactose induction of the gal enzymes; the mutant had acquired a glucose-resistant alternative transport mechanism for galactose via the constitutively synthesized galactoside permease. Further support for the transport inhibition model was provided by the finding that glucose did not substantially inhibit induction of the gal enzymes when glucose and galactose were produced intracellularly by beta-galactosidase hydrolysis of lactose, even if excess glucose was added. The inducer uptake experiments also showed that d-galactose and d-fucose probably enter the cell via different transport systems, although uptake of both compounds was inhibited by glucose.
阿迪亚，桑卡尔（威斯康星大学麦迪逊分校），以及哈里森·埃科尔斯。葡萄糖效应与大肠杆菌的半乳糖酶：葡萄糖抑制诱导与诱导物转运之间的相关性。《细菌学杂志》92:601 - 608。1966年。——在大肠杆菌中研究了葡萄糖对利用半乳糖所需酶诱导的抑制作用（“葡萄糖效应”）。对标记诱导物（d - 半乳糖 - C(14)和d - 岩藻糖 - H(3)）进入细胞的实验表明，诱导物转运水平的抑制可能是葡萄糖对半乳糖酶产生葡萄糖效应的主要机制。这一解释得到以下发现的支持：一个对乳糖酶组成型的突变体对葡萄糖抑制半乳糖酶的半乳糖诱导具有抗性；该突变体通过组成型合成的半乳糖苷通透酶获得了一种对葡萄糖有抗性的半乳糖替代转运机制。当通过β - 半乳糖苷酶水解乳糖在细胞内产生葡萄糖和半乳糖时，即使添加过量葡萄糖，葡萄糖也不会显著抑制半乳糖酶的诱导，这一发现为转运抑制模型提供了进一步支持。诱导物摄取实验还表明，d - 半乳糖和d - 岩藻糖可能通过不同的转运系统进入细胞，尽管两种化合物的摄取都受到葡萄糖的抑制。
