School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, PR China.
Planta Med. 2012 Dec;78(18):1967-71. doi: 10.1055/s-0032-1327877. Epub 2012 Oct 31.
Four novel phenolic compounds (1-4) were isolated from the leaves of Crataegus pinnatifida, along with three known ones (5-7). Their structures were elucidated as: methyl 4-O-β-D-glucopyranosyl-3-[(2E,6E)-8-O-β-D-glucopyranosyl-3,7-dimethyl-2,6-octadienyl] benzoate (1), biphenyl-5-ol-3-O-β-D-glucoside (2), 3,4'-dimethoxy-biphenyl-5-ol-4-O-β-D-glucoside (3), (E)-6-(benzoyloxy)-1-hydroxyhex-3-en-2-O-β-D-glucoside (4), shanyenoside A (5), eriodectyol (6), and 2″-O-rhamnosyl vitexin (7), using a combination of mass spectroscopy, 1D and 2D NMR spectroscopy, and chemical analysis. The antithrombotic activity of the isolated compounds was investigated on the transgenic zebra fish system. Among them, eriodectyol (6) potently inhibited the production of thrombus.
从山楂叶中分离得到了 4 种新的酚类化合物（1-4），以及 3 种已知化合物（5-7）。它们的结构被阐明为：甲基 4-O-β-D-吡喃葡萄糖基-3-[(2E,6E)-8-O-β-D-吡喃葡萄糖基-3,7-二甲基-2,6-辛二烯基]苯甲酸酯（1）、联苯-5-醇-3-O-β-D-葡萄糖苷（2）、3,4'-二甲氧基联苯-5-醇-4-O-β-D-葡萄糖苷（3）、(E)-6-(苯甲酰氧基)-1-羟基己-3-烯-2-O-β-D-葡萄糖苷（4）、山茱萸苷 A（5）、圣草酚（6）和 2″-O-鼠李糖苷牡荆素（7），使用质谱、1D 和 2D NMR 光谱以及化学分析相结合的方法。对分离得到的化合物进行了抗血栓活性研究，发现圣草酚（6）能有效抑制血栓的形成。
