Luo Meng, Yang Xuan, Hu Jiao-Yang, Jiao Jiao, Mu Fan-Song, Song Zhuo-Yue, Gai Qing-Yan, Qiao Qi, Ruan Xin, Fu Yu-Jie
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry Univ, Harbin, 150040, PR China.
Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
J Food Sci. 2016 May;81(5):C1102-9. doi: 10.1111/1750-3841.13291. Epub 2016 Apr 13.
In this study, the effect of seasonal variations on Crataegus pinnatifida, changes in antioxidant activity and active components in C. pinnatifida leaves, roots, twigs, and fruits from May to October were investigated. Through correlation analysis of climatic factors and 7 phenolic compounds yield, the phenolic compounds content was positively correlated with temperatures and daytime. The correlation coefficient of temperatures and daytime were 0.912 and 0.829, respectively. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging and reducing power tests were employed to evaluate the antioxidant activity of the C. pinnatifida. C. pinnatifida leaves exhibited significant advantages in terms of higher phenolic contents and excellent antioxidant activities. Principal component analysis (PCA) revealed that 2 main PC characterize the C. pinnatifida phenolic composition (82.1% of all variance). C. pinnatifida leaves in September possessed remarkable antioxidant activity. The results elucidate that C. pinnatifida leaves, as renewable parts, are suitable for application as antioxidant ingredients.
本研究调查了季节变化对山楂的影响，以及5月至10月期间山楂叶、根、枝和果实中抗氧化活性和活性成分的变化。通过对气候因素与7种酚类化合物产量的相关性分析，发现酚类化合物含量与温度和白昼时长呈正相关。温度和白昼时长的相关系数分别为0.912和0.829。采用1,1-二苯基-2-苦基肼（DPPH）自由基清除、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除和还原力测试来评估山楂的抗氧化活性。山楂叶在酚类含量较高和抗氧化活性优异方面表现出显著优势。主成分分析（PCA）表明，2个主要主成分表征了山楂的酚类成分（占总方差的82.1%）。9月的山楂叶具有显著的抗氧化活性。结果表明，山楂叶作为可再生部分，适合作为抗氧化成分应用。
