CNRS, Institut de Pharmacologie et de Biologie Structurale, BP 64182, 205 route de Narbonne, F-31077 Toulouse, France.
Curr Drug Metab. 2013 Mar;14(3):300-8. doi: 10.2174/1389200211314030005.
There are a number of stigmas attached to the development of antitumor drugs such as their safe and efficient delivery into target cells or tissues. The one such case is that of macromolecules, such as nucleic acids where it poses severe limits. From this point of view, electro-pulsation proves to be a promising method for cancer-associated gene therapy. It involves the direct application of electric pulses on cells or tissues which leads to a transient permeabilization of their plasma membrane allowing efficient in vitro and in vivo delivery of exogenous molecules. The present review probes the electrotransfer of nucleic acids, the nature of nucleic acids (plasmid DNA, mRNA, siRNA, LNA...) which can be electrotransferred and the mechanism of their electrotransfer.
抗肿瘤药物的发展存在许多问题，例如将其安全有效地递送到靶细胞或组织中。大分子药物就是一个例子，例如核酸药物，这给它们的应用带来了严重的限制。从这个角度来看，电脉冲被证明是一种很有前途的癌症相关基因治疗方法。它涉及直接在细胞或组织上施加电脉冲，导致其质膜瞬时通透性增加，从而实现外源性分子的高效体外和体内传递。本文综述了核酸的电转移、可电转移的核酸的性质（质粒 DNA、mRNA、siRNA、LNA……）以及它们的电转移机制。
