Lab of Mechanics, Aristotle University of Thessaloniki, Thessaloniki, Greece Department of Physics, University of the Witwatersrand, Wits, South Africa.
J Microsc. 2013 Jan;249(1):62-8. doi: 10.1111/j.1365-2818.2012.03681.x. Epub 2012 Nov 1.
Atomic force microscopy, in a liquid environment, was used to capture in vitro the morphological and mechanical changes that cultured fibroblasts undergo as time elapses from the completion of the cell culture. Topography images illustrated that initially, the nucleus had a height of 1.18 ± 0.2 μm, and after 48 h it had decreased to 550 ± 60 nm; similarly, the cell membrane exhibited significant shrinkage from 34 ± 4 to 23 ± 2 μm. After each image scan, atomic force microscopy indentation was performed on the centre of the nucleus, to measure the changes in the cell elasticity. Examination of the force-distance curves indicated that the membrane elastic modulus at the nucleus remained the same within the time frame of 48 h, even though the cell morphology had significantly changed.
原子力显微镜在液体环境中被用于体外捕获培养的成纤维细胞在完成细胞培养后随时间流逝所经历的形态和机械变化。形貌图像表明，细胞核最初的高度为 1.18 ± 0.2 μm，48 h 后降低至 550 ± 60 nm；同样，细胞膜也从 34 ± 4 μm显著收缩至 23 ± 2 μm。在每次图像扫描后，对细胞核中心进行原子力显微镜压痕测试，以测量细胞弹性的变化。力-距离曲线的检查表明，即使细胞形态发生了显著变化，核膜弹性模量在 48 h 的时间范围内仍保持不变。
