College of Veterinary Medicine, Chungnam National University, Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.
Food Chem Toxicol. 2013 Jan;51:364-9. doi: 10.1016/j.fct.2012.10.017. Epub 2012 Oct 29.
Platycodin D (PD) is the major triterpene saponin in the root of Platycodon grandiflorum. The aim of the present study was to evaluate the protective effects of PD on bile duct ligation (BDL)-induced cholestasis in mice. Mice were allocated to five groups: sham, BDL alone, and BDL with PD treatment at 1, 2, and 4mg/kg. PD was administered to the mice for 28 consecutive days after the BDL operation. PD treatment of BDL-operated mice decreased serum alanine aminotransferase, serum aspartate aminotransferase, and total bilirubin levels by up to 37%, 31%, and 41%, respectively, in comparison with the levels in mice that underwent BDL alone. PD treatment attenuated oxidative stress, as evidenced by an increase in anti-oxidative enzyme levels glutathione and superoxide dismutase together with a decrease in lipid peroxidation and oxidative stress indices levels of malondialdehyde and nitric oxide. Histopathological studies further confirmed the protective effects of PD on cholestasis-induced hepatic injury and liver fibrosis in mice. In addition, nuclear factor-kappa B and inducible nitric oxide synthase levels significantly decreased after PD treatment, as did the levels of hepatocyte apoptosis. Taken together, these results suggest that PD treatment might be beneficial in cholestasis-induced hepatotoxicity.
远志酸 D（PD）是桔梗根中的主要三萜皂苷。本研究旨在评估 PD 对胆管结扎（BDL）诱导的小鼠胆汁淤积的保护作用。将小鼠分为五组：假手术组、BDL 组、PD 治疗 1、2、4mg/kg 组。BDL 手术后，PD 连续给药 28 天。与单独 BDL 组相比，PD 治疗可使 BDL 手术小鼠的血清丙氨酸氨基转移酶、血清天冬氨酸氨基转移酶和总胆红素水平分别降低 37%、31%和 41%。PD 治疗可减轻氧化应激，表现为抗氧化酶谷胱甘肽和超氧化物歧化酶水平升高，同时脂质过氧化和氧化应激指标丙二醛和一氧化氮水平降低。组织病理学研究进一步证实了 PD 对胆汁淤积诱导的肝损伤和肝纤维化的保护作用。此外，PD 治疗后核因子-κB 和诱导型一氧化氮合酶水平显著降低，肝细胞凋亡水平也降低。综上所述，这些结果表明 PD 治疗可能有益于胆汁淤积性肝毒性。
