Department of Obstetrics & Gynaecology, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong, PR China.
J Assist Reprod Genet. 2012 Dec;29(12):1443-6. doi: 10.1007/s10815-012-9880-1. Epub 2012 Nov 2.
Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL).
A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L).
We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R(2) = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 × AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 × AMH (DSL) - 1.270 respectively.
AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended.
抗苗勒管激素（AMH）分析在生殖医学中变得越来越重要，但检测方法尚未标准化。我们评估了贝克曼库尔特的新型 Gen II ELISA 试剂盒与 Immunotech（IOT）和诊断系统实验室（DSL）的旧 ELISA 试剂盒之间的相关性。
共检索了 56 份来自患有不育或生殖内分泌疾病患者的存档血清样本，并使用三种 AMH ELISA 试剂盒进行重复检测。这些样本涵盖了广泛的 AMH 浓度范围（1.9 至 142.5 pmol/L）。
我们观察到新型（AMH Gen II）和旧 AMH 检测试剂盒与 IOT 和 DSL 之间具有良好的相关性（R²分别为 0.971 和 0.930）。回归方程分别为 AMH（Gen II）= 1.353×AMH（IOT）+0.051 和 AMH（Gen II）= 1.223×AMH（DSL）-1.270。
使用 Gen II 试剂盒的 AMH 浓度略高于 IOT 和 DSL 试剂盒。迫切需要对检测结果进行标准化，但这项分析有助于解释使用任何三种检测方法获得的历史和未来研究中的值。同时，不建议通过直接转换来自先前发表的工作的临床临界值来进行适应。
