Gavel Y, von Heijne G
Department of Theoretical Physics, Royal Institute of Technology, Stockholm, Sweden.
FEBS Lett. 1990 Feb 26;261(2):455-8. doi: 10.1016/0014-5793(90)80614-o.
A collection of 32 stroma-targeting chloroplast transit peptides with known cleavage sites have been analysed in terms of amino acid preferences in the vicinity of the processing site. A loosely conserved consensus motif (Val/Ile)-X-(Ala/Cys) decreases Ala is found in the majority of the transit peptides. About 30% of the sequences have a perfect match to the consensus. When such a match is found, there is a 90% probability that it specifies the correct cleavage site.
对32个具有已知切割位点的靶向基质的叶绿体转运肽进行了分析，以研究加工位点附近的氨基酸偏好。一个松散保守的共有基序（Val/Ile）-X-（Ala/Cys）在大多数转运肽中发现Ala减少。约30%的序列与共有序列完全匹配。当发现这种匹配时，有90%的概率它指定了正确的切割位点。
