Levi-Schaffer F, Bar-Shavit Z
Department of Pharmacology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Immunology. 1990 Jan;69(1):145-9.
We studied the ability of the rat osteosarcoma derived cell-line with osteoblastic properties ROS-17/2.8 (ROS) to maintain in vitro rat peritoneal mast cells (MC) in a functional state. Highly purified (greater than 95%) MC were seeded on confluent ROS cells. The MC adhered tightly to the monolayers within a few hours and remained viable for at least 2 weeks, but did not proliferate. The MC retained their typical appearance, exhibiting highly granulated resting morphology when stained with alcian blue followed by safranin or with acidic toluidine blue. Furthermore, after 2 weeks, the MC were fully responsive to activation with compound 48/80 (3 micrograms/ml), releasing 75% of their histamine content, as compared to 3% in the absence of the secretagogue. Utilizing metabolically inactive ROS cells and prevention of contact between ROS and MC, we found that both release of factor(s) and cell-cell contact were required by ROS to exhibit their MC supporting activity. Various other cells and cell lines were unable to support MC viability. On the other hand, as demonstrated before, 3T3 fibroblasts were capable of promoting MC viability. Thus, MC viability and functional activity are specifically maintained by fibroblastic and osteoblastic cells. The abundance of MC in bone, and their participation in bone remodelling raise the possibility of physiological and pathological significance to interactions between MC and osteoblasts.
我们研究了具有成骨细胞特性的大鼠骨肉瘤衍生细胞系ROS-17/2.8（ROS）在体外维持大鼠腹膜肥大细胞（MC）功能状态的能力。将高度纯化（大于95%）的MC接种到汇合的ROS细胞上。MC在数小时内紧密粘附于单层细胞，并且至少存活2周，但不增殖。MC保持其典型外观，在用阿尔辛蓝接着番红染色或用酸性甲苯胺蓝染色时呈现高度颗粒状的静息形态。此外，2周后，MC对化合物48/80（3微克/毫升）的激活完全有反应，释放其75%的组胺含量，相比之下，在没有促分泌剂的情况下为3%。利用代谢不活跃的ROS细胞以及防止ROS与MC之间的接触，我们发现ROS表现出其MC支持活性既需要因子释放也需要细胞间接触。各种其他细胞和细胞系均无法支持MC的存活。另一方面，如之前所证明的，3T3成纤维细胞能够促进MC的存活。因此，MC的存活和功能活性由成纤维细胞和成骨细胞特异性维持。骨中MC的丰富以及它们参与骨重塑增加了MC与成骨细胞之间相互作用具有生理和病理意义的可能性。
