Bar-Shavit Z, Kahn A J, Stone K R, Trial J, Hilliard T, Reitsma P H, Teitelbaum S L
Endocrinology. 1986 Feb;118(2):679-86. doi: 10.1210/endo-118-2-679.
HL-60 cells are induced to differentiate along a monocytic pathway by the active metabolites of vitamin D3, e.g. 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. All such differentiated cells share a number of features in common but are heterogeneous in their ability to adhere to solid substrates and to resorb devitalized bone matrix. Here, we show that, in addition, as compared to the nonadherent, adherent cells are smaller, less likely to be in the S phase, more enriched in the human monocyte-specific cell surface antigen, 63D3, and contain less cmyc messenger RNA (mRNA). In addition, we document that removal of the hormone leads to dedifferentiation. For these susceptible mononuclear cells, removal of 1,25-(OH)2D3 results in a reversion to a more myeloblastic phenotype, renewed cell proliferation, and the rapid appearance of elevated levels of cmyc mRNA. Finally, we report that the cells that do not revert upon 1,25-(OH)2D3 removal are those that became multinucleated during treatment.
维生素D3的活性代谢产物，如1,25 - 二羟基维生素D3 [1,25-(OH)2D3]，可诱导HL - 60细胞沿单核细胞途径分化。所有这些分化细胞都有一些共同特征，但在粘附于固体基质和吸收失活骨基质的能力方面存在异质性。在此，我们表明，此外，与不粘附细胞相比，粘附细胞更小，处于S期的可能性更低，人类单核细胞特异性细胞表面抗原63D3的富集程度更高，且c - myc信使核糖核酸（mRNA）含量更少。此外，我们证明去除激素会导致去分化。对于这些易感单核细胞，去除1,25-(OH)2D3会导致细胞恢复为更具成髓细胞特征的表型，细胞增殖重新开始，且c - myc mRNA水平迅速升高。最后，我们报告称，去除1,25-(OH)2D3后未发生逆转的细胞是那些在治疗过程中形成多核的细胞。
