构建基于 Tn7 的广谱载体，用于革兰氏阴性菌中单拷贝 P(BAD)-控制的基因表达。
Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.
机构信息
Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA.
出版信息
Appl Environ Microbiol. 2013 Jan;79(2):718-21. doi: 10.1128/AEM.02926-12. Epub 2012 Nov 2.
Abstract
We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.
摘要
我们描述了一种基于 mini-Tn7 的广泛宿主范围表达盒，用于 P(BAD) 启动子的阿拉伯糖诱导基因表达。该递送载体 pTJ1 可将单个基因整合到革兰氏阴性菌的染色体中，用于多种遗传应用，其中一些应用使用铜绿假单胞菌作为模型宿主进行了讨论。
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2
Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii.
J Microbiol Methods. 2010 Sep;82(3):296-300. doi: 10.1016/j.mimet.2010.07.002. Epub 2010 Jul 16.
3
Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.
FEMS Microbiol Lett. 2009 Sep;298(1):111-7. doi: 10.1111/j.1574-6968.2009.01707.x. Epub 2009 Jun 26.
4
Single-copy chromosomal integration systems for Francisella tularensis.
Microbiology (Reading). 2009 Apr;155(Pt 4):1152-1163. doi: 10.1099/mic.0.022491-0.
5
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
J Bacteriol. 2009 Apr;191(7):2285-95. doi: 10.1128/JB.01490-08. Epub 2009 Jan 23.
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PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria.
Appl Environ Microbiol. 2008 Dec;74(23):7422-6. doi: 10.1128/AEM.01369-08. Epub 2008 Oct 10.
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Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei.
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8
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9
mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344.
Nat Protoc. 2006;1(1):162-9. doi: 10.1038/nprot.2006.25.
10
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.
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