一种经过改进的Tn7-lux报告基因，用于革兰氏阴性菌中广泛宿主范围的染色体整合启动子融合。
An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.
作者信息
Glassing Angela, Lewis Thomas A
机构信息
Department of Biological and Physical Sciences, Montana State University Billings, Billings, MT 59101, United States.
Department of Biological and Physical Sciences, Montana State University Billings, Billings, MT 59101, United States.
出版信息
J Microbiol Methods. 2015 Nov;118:75-7. doi: 10.1016/j.mimet.2015.08.016. Epub 2015 Sep 1.
Abstract
An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.
摘要
描述了一种用于染色体整合型启动子 - 荧光素酶融合的改进载体。使用特征明确的大肠杆菌araBAD启动子，在铜绿假单胞菌attTn7位点将修饰后的载体与未修饰的载体进行平行测试。修饰后的mini-Tn7显示背景发光降低，诱导后发光增加，诱导比提高了16倍以上。
相似文献
1
An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.
J Microbiol Methods. 2015 Nov;118:75-7. doi: 10.1016/j.mimet.2015.08.016. Epub 2015 Sep 1.
2
Tn5/7-lux: a versatile tool for the identification and capture of promoters in gram-negative bacteria.
BMC Microbiol. 2015 Feb 4;15(1):17. doi: 10.1186/s12866-015-0354-3.
3
Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.
Appl Environ Microbiol. 2013 Jan;79(2):718-21. doi: 10.1128/AEM.02926-12. Epub 2012 Nov 2.
4
Construction of a Tn7-lux system for gene expression studies in gram-negative bacteria.
Gene. 1992 Dec 1;122(1):27-34. doi: 10.1016/0378-1119(92)90028-n.
5
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria.
J Microbiol Methods. 2013 Apr;93(1):9-11. doi: 10.1016/j.mimet.2013.01.017.
6
Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
Appl Environ Microbiol. 2013 Jul;79(13):4149-53. doi: 10.1128/AEM.00640-13. Epub 2013 Apr 12.
7
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.
Nat Protoc. 2006;1(1):153-61. doi: 10.1038/nprot.2006.24.
8
A Modular, Tn7-Based System for Making Bioluminescent or Fluorescent Salmonella and Escherichia coli Strains.
Appl Environ Microbiol. 2016 Jul 29;82(16):4931-43. doi: 10.1128/AEM.01346-16. Print 2016 Aug 15.
9
Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria.
BMC Microbiol. 2015 Sep 29;15:190. doi: 10.1186/s12866-015-0521-6.
10
Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria.
Plasmid. 1997;38(1):35-51. doi: 10.1006/plas.1997.1294.
引用本文的文献
1
Coordinates the Expression of two Manganese Oxidases and Optimizes Manganese Oxide Precipitation in Response to Aqueous Mn(II).
Environ Sci Technol. 2025 Jul 15;59(27):13777-13786. doi: 10.1021/acs.est.5c00255. Epub 2025 Jun 5.
2
Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of .
Microorganisms. 2021 Feb 12;9(2):369. doi: 10.3390/microorganisms9020369.
3
From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization.
Environ Microbiol. 2021 Apr;23(4):2070-2085. doi: 10.1111/1462-2920.15296. Epub 2021 Jan 22.
4
NADH Dehydrogenases in Growth and Virulence.
Front Microbiol. 2019 Feb 5;10:75. doi: 10.3389/fmicb.2019.00075. eCollection 2019.
本文引用的文献
1
Chromosome position effects on gene expression in Escherichia coli K-12.
Nucleic Acids Res. 2014 Oct;42(18):11383-92. doi: 10.1093/nar/gku828. Epub 2014 Sep 10.
2
Role for ferredoxin:NAD(P)H oxidoreductase (FprA) in sulfate assimilation and siderophore biosynthesis in Pseudomonads.
J Bacteriol. 2013 Sep;195(17):3876-87. doi: 10.1128/JB.00528-13. Epub 2013 Jun 21.
3
Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
Appl Environ Microbiol. 2013 Jul;79(13):4149-53. doi: 10.1128/AEM.00640-13. Epub 2013 Apr 12.
4
Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.
Appl Environ Microbiol. 2013 Jan;79(2):718-21. doi: 10.1128/AEM.02926-12. Epub 2012 Nov 2.
5
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.
Nat Protoc. 2006;1(1):153-61. doi: 10.1038/nprot.2006.24.
6
A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site.
J Microbiol Methods. 2001 Jul;45(3):187-95. doi: 10.1016/s0167-7012(01)00246-9.
7
Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.
Gene. 1999 Feb 18;227(2):197-203. doi: 10.1016/s0378-1119(98)00601-5.
8
Bacterial bioluminescence: organization, regulation, and application of the lux genes.
FASEB J. 1993 Aug;7(11):1016-22. doi: 10.1096/fasebj.7.11.8370470.
9
Construction of a Tn7-lux system for gene expression studies in gram-negative bacteria.
Gene. 1992 Dec 1;122(1):27-34. doi: 10.1016/0378-1119(92)90028-n.
Zotero 插件