School of Life Sciences and Institute for Microorganisms, Kyungpook National University, Daegu 702-701, Korea.
J Microbiol Biotechnol. 2012 Nov;22(11):1568-74. doi: 10.4014/jmb.1202.02047.
Microbiological calcium carbonate precipitation (MCCP) has been investigated for its ability to improve the durability of cement mortar. However, very few strains have been applied to crack remediation and strengthening of cementitious materials. In this study, we report the biodeposition of Bacillus subtilis 168 and its ability to enhance the durability of cement material. B. subtilis 168 was applied to the surface of cement specimens. The results showed a new layer of deposited organic-inorganic composites on the surface of the cement paste. In addition, the water permeability of the cement paste treated with B. subtilis 168 was lower than that of non-treated specimens. Furthermore, artificial cracks in the cement paste were completely remediated by the biodeposition of B. subtilis 168. The compressive strength of cement mortar treated with B. subtilis 168 increased by about 19.5% when compared with samples completed with only B4 medium. Taken together, these findings suggest that the biodeposition of B. subtilis 168 could be used as a sealing and coating agent to improve the strength and water resistance of concrete. This is the first paper to report the application of Bacillus subtilis 168 for its ability to improve the durability of cement mortar through calcium carbonate precipitation.
微生物碳酸钙沉淀（MCCP）因其能够提高水泥砂浆耐久性而受到研究。然而，很少有菌株被应用于裂缝修复和增强水泥基材料。在本研究中，我们报告了枯草芽孢杆菌 168 的生物沉积及其增强水泥材料耐久性的能力。枯草芽孢杆菌 168 被应用于水泥试件的表面。结果表明，在水泥浆体表面形成了一层新的沉积有机-无机复合材料。此外，经枯草芽孢杆菌 168 处理的水泥浆体的水渗透性低于未经处理的试样。此外，枯草芽孢杆菌 168 的生物沉积完全修复了水泥浆体中的人工裂缝。与仅用 B4 培养基完成的样品相比，用枯草芽孢杆菌 168 处理的水泥砂浆的抗压强度提高了约 19.5%。综上所述，这些发现表明，枯草芽孢杆菌 168 的生物沉积可以用作密封和涂层剂，以提高混凝土的强度和耐水性。这是第一篇报道枯草芽孢杆菌 168 通过碳酸钙沉淀提高水泥砂浆耐久性的应用的论文。
