Ahmed Shiren O, Nasser Amal A, Abbas Rateb N, Kamal Monir M, Zahran Magdy A, Sorour Noha M
Department of Civil Engineering, Delta Higher Institute for Engineering and Technology, Mansoura, Egypt.
Department of Civil Engineering, Faculty of Engineering, Minufyia University, Sadat, 22857/79 Egypt.
3 Biotech. 2021 May;11(5):231. doi: 10.1007/s13205-021-02781-0. Epub 2021 Apr 22.
Microbial-based self-healing of concrete represents innovative technology for improving micro-crack sealing. Microbial bioactivity can induce calcite-precipitation in concrete, which seals micro-cracks. In this respect, two Egyptian bacterial isolates were selected and identified, as (Bs) and (Bm) using MALDI-TOF/MS-Biotyper. Peak patterns of the bacterial ribosomal proteins showed a high match between samples and standards, which verified species consistency. Bs and Bm were added to the mortar mixture in two concentrations (0.5%, 1%) of cement weight, then the mechanical and physical properties were tested throughout a 180-day time course. The compressive strength of Bm0.5 bacterial mortar samples was increased by 21.4% after 28 days, as compared to control. The rate of water absorption of Bm samples was decreased by 12.4% after 180 days. Bacterial mortar samples showed significant restoration of compressive strength than the original samples by 44%, 21%, and 52.6% for Bs1, Bm0.5, and Bs0.5, respectively. SEM and EDAX analyses confirmed that bacterial samples were denser with fewer voids than the control, as a result of microbial nanosized calcite-precipitation. DTA verified that the amount of CaCO and its degree of crystallinity were increased in the bacterial mortar samples. Load-deflection of reinforced-laminates for bacterial mortar samples showed ductile behavior and less deformation as compared to control. In this work, novel concrete with improved mechanical and physical properties has been developed using selected Egyptian microorganisms, it can promote self-healing of micro-cracks with improved durability of the concrete. The application of self-healing bioconcrete can reduce the inspection and maintenance costs.
The online version contains supplementary material available at 10.1007/s13205-021-02781-0.
基于微生物的混凝土自修复是一种用于改善微裂缝密封的创新技术。微生物的生物活性可诱导混凝土中方解石沉淀,从而密封微裂缝。在这方面,使用基质辅助激光解吸电离飞行时间质谱生物分型仪(MALDI-TOF/MS-Biotyper)选择并鉴定了两种埃及细菌分离株,分别为(Bs)和(Bm)。细菌核糖体蛋白的峰型显示样品与标准品高度匹配,证实了物种一致性。将Bs和Bm以水泥重量的两种浓度(0.5%、1%)添加到砂浆混合物中,然后在180天的时间过程中测试其力学和物理性能。与对照相比,Bm0.5细菌砂浆样品在28天后抗压强度提高了21.4%。Bm样品在180天后吸水率降低了12.4%。细菌砂浆样品的抗压强度比原始样品有显著恢复,Bs1、Bm0.5和Bs0.5分别恢复了44%、21%和52.6%。扫描电子显微镜(SEM)和能量色散X射线光谱(EDAX)分析证实,由于微生物纳米级方解石沉淀,细菌样品比对照更致密,孔隙更少。差示热分析(DTA)证实细菌砂浆样品中方解石的含量及其结晶度增加。与对照相比,细菌砂浆样品的增强层压板的荷载-挠度表现出延性行为且变形较小。在这项工作中,利用选定的埃及微生物开发出了具有改善的力学和物理性能的新型混凝土,它可以促进微裂缝的自修复并提高混凝土的耐久性。自修复生物混凝土的应用可以降低检测和维护成本。
在线版本包含可在10.1007/s13205-021-02781-0获取的补充材料。