Rodrigues M R, Lennette D A, Arentsen J J, Thompson C
Ophthalmology. 1979 Mar;86(3):452-64. doi: 10.1016/s0161-6420(79)35507-5.
Recent methods for detection of viruses in clinical specimens include immunofluorescence, immunoperoxidase, immune adherence hemagglutination, radioimmunoassay, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy. Some are useful for the detection of traces of viral antigens but are more complicated and time-consuming than others. Simple techniques of immunofluorescence and negative stain electron microscopy are used for the rapid detection of viruses in human adenoviral, herpetic, rubella, molluscum contagiosum, and vaccinial infections.
目前用于检测临床标本中病毒的方法包括免疫荧光法、免疫过氧化物酶法、免疫黏附血凝法、放射免疫测定法、酶联免疫吸附测定法(ELISA)和免疫电子显微镜法。有些方法可用于检测痕量病毒抗原,但比其他方法更复杂且耗时。免疫荧光和负染色电子显微镜的简单技术可用于快速检测人类腺病毒、疱疹病毒、风疹病毒、传染性软疣病毒和牛痘病毒感染中的病毒。