Doane F W, Anderson N, Chao J, Noonan A
Appl Microbiol. 1974 Feb;27(2):407-10. doi: 10.1128/am.27.2.407-410.1974.
An embedding method requiring only 2 h to complete and giving excellent ultrastructural preservation has been used for the rapid detection of viruses in tissue cultures. The method has also been applied successfully to mammalian tissue. It provides a rapid technique for identifying viruses isolated in tissue cultures, for screening cultures for adventitious agents, and for examining tissue biopsies for viruses.
一种仅需2小时即可完成且能实现超微结构完美保存的包埋方法已被用于在组织培养物中快速检测病毒。该方法也已成功应用于哺乳动物组织。它为鉴定在组织培养物中分离出的病毒、筛查培养物中的外来因子以及检查组织活检样本中的病毒提供了一种快速技术。