Perry M L, Kayes S G, Barnard J W, Taylor A E
Department of Physiology, University of South Alabama College of Medicine, Mobile 36688.
J Appl Physiol (1985). 1990 Jan;68(1):235-40. doi: 10.1152/jappl.1990.68.1.235.
Human blood was separated into polymorphonuclear (PMN) and mononuclear (MN) leukocyte fractions, and 3 x 10(7) cells (PMN or MN) were added to isolated rat lungs perfused with 5% human albumin in buffer and stimulated with phorbol myristate acetate (PMA). Lungs perfused with either albumin alone, PMN, or MN but not stimulated with PMA showed no change in vascular resistance or endothelial permeability measured as the capillary filtration coefficient (Kf,c). Lungs that were stimulated with PMA with no cells showed no change in Kf,c (0.34 +/- 0.07 vs. 0.37 +/- 0.7), but vascular resistance increased in all segments of the circulation. Capillary pressure, the major force responsible for edema formation, nearly doubled in the absence of cells 40 min after PMA. Lungs perfused with either PMN or MN and stimulated with PMA were injured. Kf,c increased from 0.41 +/- 0.03 to 0.87 +/- 0.10 (PMN) and from 0.36 +/- 0.07 to 0.81 +/- 0.23 (MN) 90 min after PMA. In addition to the increased endothelial permeability, vascular resistances and pressures also increased in the cell-perfused PMA-stimulated lungs. These results demonstrate that cells other than granulocytes are capable of producing severe acute lung injury and cannot be ignored when the effects of PMA on neutrophil-depleted lungs are studied.
将人血分离为多形核（PMN）和单核（MN）白细胞组分，将3×10⁷个细胞（PMN或MN）加入到用含5%人白蛋白的缓冲液灌注的离体大鼠肺中，并用佛波酯（PMA）刺激。单独用白蛋白、PMN或MN灌注但未用PMA刺激的肺，以毛细血管滤过系数（Kf,c）衡量的血管阻力或内皮通透性无变化。未加细胞而用PMA刺激的肺，Kf,c无变化（0.34±0.07对0.37±0.7），但循环各段的血管阻力均增加。在PMA作用40分钟后无细胞的情况下，导致水肿形成的主要力量——毛细血管压力几乎增加了一倍。用PMN或MN灌注并经PMA刺激的肺受到损伤。PMA作用90分钟后，Kf,c从0.41±0.03增加到0.87±0.10（PMN组），从0.36±0.07增加到0.81±0.23（MN组）。除了内皮通透性增加外，在细胞灌注并经PMA刺激的肺中，血管阻力和压力也增加。这些结果表明，除粒细胞外的其他细胞也能够造成严重的急性肺损伤，因此在研究PMA对中性粒细胞减少的肺的影响时不能忽视。
