Perry M, Taylor A E
Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688.
J Appl Physiol (1985). 1988 Nov;65(5):2164-9. doi: 10.1152/jappl.1988.65.5.2164.
The effect of leukocyte depletion on acute lung injury produced by intravenous or intratracheal phorbol myristate acetate (PMA) administration was studied in isolated perfused rat lungs. Vascular endothelial permeability was assessed by use of the capillary filtration coefficient (Kf,c). A predicted pulmonary capillary pressure (Ppc,p) was calculated from measurements of postcapillary resistances. These parameters were measured before and 90 min after the administration of PMA, either intratracheally or intravascularly. When blood elements were present both intratracheal and intravascular PMA caused an increased Kf,c [0.27 +/- 0.02 vs. 0.99 +/- 0.22 and 0.25 +/- 0.05 vs. 0.64 +/- 0.15 (SE) ml.min-1.cmH2O-1.100 g-1, respectively; P less than 0.05] and an increased Ppc,p (8.3 +/- 0.4 vs. 74.7 +/- 18.3 and 8.7 +/- 0.8 vs. 74.2 +/- 25.1 cmH2O, respectively; P less than 0.05). Removal of circulating leukocytes abolished the increased Kf,c when PMA was given intratracheally (0.35 +/- 0.06 vs. 0.23 +/- 0.07 ml.min-1.cmH2O-1.100 g-1) or intravascularly (0.39 +/- 0.07 vs. 0.33 +/- 0.07 ml.min-1.cmH2O-1.100 g-1). In the absence of neutrophils, Ppc,p slightly increased with intratracheal PMA, from 6.9 +/- 0.5 to 10.5 +/- 1.1 cmH2O (P less than 0.05), but was unchanged at 90 min with intravascular PMA. Depletion of circulating neutrophils with an antineutrophil serum failed to block the Kf,c change with intratracheal PMA (from 0.24 +/- 0.03 to 0.42 +/- 0.09 ml.min-1.cmH2O-1.100 g-1; P less than 0.05). Ppc,p also increased from 6.9 +/- 0.6 to 19.8 +/- 6.7 cmH2O (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
在离体灌注的大鼠肺中研究了白细胞清除对静脉内或气管内给予佛波酯肉豆蔻酸酯（PMA）所产生的急性肺损伤的影响。通过毛细血管滤过系数（Kf,c）评估血管内皮通透性。根据毛细血管后阻力的测量值计算预测的肺毛细血管压力（Ppc,p）。在气管内或血管内给予PMA之前及之后90分钟测量这些参数。当气管内和血管内均存在血液成分时，气管内和血管内给予PMA均导致Kf,c升高[分别为0.27±±0.02与0.99±±0.22以及0.25±±0.05与0.64±±0.15（SE）ml·min-1·cmH2O-1·100 g-1；P<0.05]以及Ppc,p升高（分别为8.3±±0.4与74.7±±18.3以及8.7±±0.8与74.2±±25.1 cmH2O；P<0.05）。当气管内给予PMA时（0.35±±与0.23±±0.07 ml·min-1·cmH2O-1·100 g-1）或血管内给予PMA时（0.39±±0.07与0.33±±0.07 ml·min-1·cmH2O-1·100 g-1），清除循环白细胞可消除Kf,c的升高。在无中性粒细胞的情况下，气管内给予PMA时Ppc,p略有升高，从6.9±±0.5升至10.5±±（P<0.05），但血管内给予PMA 90分钟时Ppc,p无变化。用抗中性粒细胞血清清除循环中性粒细胞未能阻止气管内给予PMA时Kf,c的变化（从0.24±±0.03至0.42±±0.09 ml·min-1·cmH2O-1·100 g-1；P<0.05）。Ppc,p也从6.9±±0.6升至19.8±±6.7 cmH O（P<0.05）。（摘要截短于250字）
