Department of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, China.
Mediators Inflamm. 2012;2012:751629. doi: 10.1155/2012/751629. Epub 2012 Oct 16.
Free radicals and proinflammatory cytokines have been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). Picroliv, a Picrorhiza kurroa derivative, has been demonstrated to have antioxidant and anti-inflammatory effect. The purpose of the study was to investigate the effects of picroliv on experimental model of UC in mice.
Picroliv was administrated orally by gavage to mice with colitis induced by dextran sulfate sodium (DSS). Disease activity index (DAI), colon length, and histology score were observed. Myeloperoxidase (MPO) activity, and SOD, MDA concentrations were measured by enzyme-linked immunosorbent assay (ELISA) while the expression of cytokine mRNAs was studied by real-time-quantitative polymerase chain reaction and also ELISA. The expression of NF-κB p65 was observed by immunohistochemistry staining and western blotting.
A significant improvement was observed in DAI and histological score in mice treated with picroliv, and incerased MPO activity, MDA concentrations, and the expression of IL-1β, TNF-α, and NF-κB p65 in mice with DSS-induced colitis were significantly reduced while decreased SOD level increased following administration of picroliv.
The administration of picroliv leads to an amelioration of DSS-induced colitis, suggesting administration of picroliv may provide a therapeutic approach for UC.
自由基和促炎细胞因子已被证明在溃疡性结肠炎（UC）的发病机制中起关键作用。水飞蓟宾，一种土大黄提取物，已被证明具有抗氧化和抗炎作用。本研究的目的是研究水飞蓟宾对葡聚糖硫酸钠（DSS）诱导的 UC 小鼠模型的影响。
通过灌胃给予水飞蓟宾治疗 DSS 诱导的结肠炎小鼠。观察疾病活动指数（DAI）、结肠长度和组织学评分。通过酶联免疫吸附试验（ELISA）测量髓过氧化物酶（MPO）活性和 SOD、MDA 浓度，通过实时定量聚合酶链反应和 ELISA 研究细胞因子 mRNA 的表达。通过免疫组织化学染色和 Western blot 观察 NF-κB p65 的表达。
水飞蓟宾治疗的小鼠 DAI 和组织学评分明显改善，DSS 诱导的结肠炎小鼠 MPO 活性、MDA 浓度、IL-1β、TNF-α 和 NF-κB p65 的表达明显降低，而 SOD 水平降低，MDA 浓度升高。
水飞蓟宾的给药导致 DSS 诱导的结肠炎得到改善，表明水飞蓟宾的给药可能为 UC 提供一种治疗方法。
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