College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Agricultural Bioengineering Institute, Changsha 410128, China.
Nutrients. 2024 Jan 2;16(1):153. doi: 10.3390/nu16010153.
(CB) and (PI) have anti-inflammatory, immune regulation, anti-tumor, and other functions. This study aimed to explore the therapeutic effect of CB and mycelium of PI (MPI) alone and in combination on colitis mice induced by dextran sodium sulfate (DSS). Mice were randomly assigned to five groups: (1) control (CTRL), (2) DSS, (3) CB, (4) MPI, and (5) CB + MPI (CON). The weight of the mice was recorded daily during the experiment, and the length of the colon was measured on the last day of the experiment. The colons were collected for hematoxylin and eosin staining, colon contents were collected for intestinal flora analysis, and serum was collected for metabolite analysis. The results showed that compared with the DSS group, CB, MPI, and CON treatments inhibited the weight loss and colon length shortening caused by DSS, significantly increased the concentrations of interleukin (IL)-4, IL-10, and superoxide dismutase, and significantly decreased the concentrations of IL-6, tumor necrosis factor-α, and myeloperoxidase. Gene sequence analysis of 16S rRNA showed that CB, MPI, and CON treatments changed the composition and structure of intestinal microorganisms. Metabolome results showed that CB, MPI, and CON treatments changed serum metabolites in DSS-treated mice, including dodecenoylcarnitine, L-urobilinogen, and citric acid. In conclusion, CB, MPI, and CON treatments alleviated DSS-induced colitis in mice by regulating intestinal flora and metabolites, with the CON group having the best effect.
（CB）和（PI）具有抗炎、免疫调节、抗肿瘤等功能。本研究旨在探讨 CB 和 PI 菌丝体（MPI）单独及联合应用对葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的治疗作用。小鼠随机分为五组：（1）对照组（CTRL），（2）DSS 组，（3）CB 组，（4）MPI 组，和（5）CB+MPI（CON）组。实验过程中每天记录小鼠体重，实验最后一天测量结肠长度。收集结肠进行苏木精-伊红染色，收集结肠内容物进行肠道菌群分析，收集血清进行代谢物分析。结果表明，与 DSS 组相比，CB、MPI 和 CON 治疗抑制了 DSS 引起的体重减轻和结肠缩短，显著增加了白细胞介素（IL）-4、IL-10 和超氧化物歧化酶的浓度，显著降低了 IL-6、肿瘤坏死因子-α和髓过氧化物酶的浓度。16S rRNA 基因序列分析显示，CB、MPI 和 CON 治疗改变了肠道微生物的组成和结构。代谢组学结果表明，CB、MPI 和 CON 治疗改变了 DSS 处理小鼠的血清代谢物，包括十二烯酰肉碱、L-尿胆素原和柠檬酸。总之，CB、MPI 和 CON 治疗通过调节肠道菌群和代谢物缓解了 DSS 诱导的结肠炎小鼠的炎症，其中 CON 组的效果最佳。
