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双标记脂肪来源干细胞归巢至小鼠颈动脉损伤的长期 MRI 跟踪。

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury.

机构信息

Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

出版信息

Int J Nanomedicine. 2012;7:5191-203. doi: 10.2147/IJN.S35647. Epub 2012 Oct 2.

DOI:10.2147/IJN.S35647
PMID:23125528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3487538/
Abstract

BACKGROUND: Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury. METHODS: GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation. RESULTS: GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging. CONCLUSION: Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.

摘要

背景：干细胞疗法在受损或患病组织的再生修复方面显示出巨大的潜力。脂肪来源的干细胞（ADSCs）已成为细胞治疗中越来越有吸引力的候选者。磁共振成像已被证明可有效追踪经磁标记的细胞，并在细胞移植后评估其临床相关性。本研究探讨了用超顺磁性氧化铁颗粒标记绿色荧光蛋白表达的脂肪来源干细胞（GFP-ADSCs）并在细胞移植后用非侵入性磁共振成像在小鼠颈动脉损伤模型中对其进行体内示踪的可行性。

方法：从 GFP 小鼠的脂肪组织中分离 GFP-ADSCs 并用超顺磁性氧化铁颗粒进行标记。体外评估标记细胞的细胞内稳定性、增殖和活力。然后，将细胞移植到小鼠颈动脉损伤模型中。在细胞移植前及 1、3、7、14、21 和 30 天后立即进行临床 3T 磁共振成像。在移植后 7 和 30 天进行普鲁士蓝染色和组织学分析。

结果：GFP-ADSCs 被超顺磁性氧化铁颗粒高效标记，对活力和增殖没有影响。磁共振成像可监测标记细胞归巢到受损颈动脉组织。

结论：表达 GFP 的磁性标记 ADSCs 可归巢到血管损伤部位，可能为理解心血管病变的基于细胞的治疗提供新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/219391f0fcb7/ijn-7-5191f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/5295c075b9b9/ijn-7-5191f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/688ccb40ec2a/ijn-7-5191f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/2fd39588ebfd/ijn-7-5191f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/07cbbc395001/ijn-7-5191f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/7746d155c061/ijn-7-5191f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/bb31ce414ed2/ijn-7-5191f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/84b6057e7a56/ijn-7-5191f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/f7809972d87b/ijn-7-5191f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/219391f0fcb7/ijn-7-5191f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/5295c075b9b9/ijn-7-5191f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/688ccb40ec2a/ijn-7-5191f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/2fd39588ebfd/ijn-7-5191f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/07cbbc395001/ijn-7-5191f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/7746d155c061/ijn-7-5191f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/bb31ce414ed2/ijn-7-5191f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/84b6057e7a56/ijn-7-5191f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/f7809972d87b/ijn-7-5191f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/410a/3487538/219391f0fcb7/ijn-7-5191f9.jpg

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[7]
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