粗球孢子菌补体结合抗原和沉淀素抗原的纯化及氨基末端序列分析
Purification and amino-terminal sequence analysis of the complement-fixing and precipitin antigens from Coccidioides immitis.
作者信息
Resnick S, Zimmer B, Pappagianis D, Eakin A, McKerrow J
机构信息
Department of Dermatology, University of California, San Francisco 94143.
出版信息
J Clin Microbiol. 1990 Feb;28(2):385-8. doi: 10.1128/jcm.28.2.385-388.1990.
Two proteins (21 and 48 kilodaltons) purified from endospore-spherule culture filtrates of Coccidioides immitis are identified as precipitin and complement-fixing antigens, respectively. To allow specific structural comparison to antigens identified by other investigators and as a first step to eventual serodiagnostic antigen production by recombinant DNA technology, amino-terminal amino acid sequences were determined for these antigens.
从粗球孢子菌的内生孢子-球形体培养滤液中纯化出的两种蛋白质（分别为21千道尔顿和48千道尔顿），被分别鉴定为沉淀素抗原和补体结合抗原。为了能与其他研究者鉴定出的抗原进行特定的结构比较，并作为最终通过重组DNA技术生产血清诊断抗原的第一步，我们测定了这些抗原的氨基末端氨基酸序列。
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