用单特异性抗血清通过免疫亲和层析法分离粗球孢子菌F抗原。
Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum.
作者信息
Cox R A, Britt L A, Michael R A
出版信息
Infect Immun. 1987 Jan;55(1):227-32. doi: 10.1128/iai.55.1.227-232.1987.
Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody to the purified antigen. The availability of these reagents will enable the development of a sensitive and specific assay for detecting serologic reactivity to this antigen.
检测针对粗球孢子菌F抗原的抗体在球孢子菌病的诊断中已被证明具有价值。通过使用针对粗球孢子菌素抗原和抗粗球孢子菌抗血清的免疫扩散试验可检测到这种抗体。我们利用凝集素亲和与免疫亲和层析相结合的方法，从粗球孢子菌素中分离出F抗原，并制备了针对纯化抗原的单特异性抗体。这些试剂的可得将有助于开发一种灵敏且特异的检测方法，用于检测针对该抗原的血清学反应性。
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