肉毒毒素 A 腺体内注射减少兔的泪液分泌。
Intraglandular injection of botulinum toxin a reduces tear production in rabbits.
机构信息
Department of Ophthalmology, The Wilmer Eye Institute, The Wilmer Eye Institute, The Johns Hopkins Hospital, Baltimore, Maryland, USA.
出版信息
Ophthalmic Plast Reconstr Surg. 2013 Jan-Feb;29(1):21-4. doi: 10.1097/IOP.0b013e31826e8a86.
PURPOSE
To develop an animal model and investigate the dose-dependent effect of an intraglandular injection of botulinum toxin A (BTX-A) on tear production.
METHODS
In a volume of 0.1-ml, 0.625-, 1.25-, or 2.5-U BTX-A was injected transconjunctivally in the superolateral lobe of the lacrimal gland of adult New Zealand white female rabbits. In the contralateral lacrimal gland, 0.1 ml of 0.9% sodium chloride was injected. Prior to injection and at 1-week postinjection, photographs were taken to evaluate pre- and postoperative eyelid position. Fluorescein and Rose Bengal stain were used to evaluate the corneal surface, and Schirmer test was used to assess tear production.
RESULTS
Glands injected with the intermediate (1.25 U) and the highest (2.5 U) doses of BTX-A displayed a statistically significant decrease in tear production (p = 0.002 and 0.007, respectively) compared with the contralateral saline-injected glands at 1 week. No corneal pathologic factors from excessive dryness were observed following the injection. While postinjection ptosis was observed (p = 0.025), no difference was seen between BTX-A and saline-injected eyes.
CONCLUSIONS
In rabbits, intraglandular injection of BTX-A resulted in decreased tear production at 1 week. No additional reduction in tear production was seen with a BTX-A dose greater than 1.25 U, suggesting glandular receptor saturation at this dose. Despite suppression of tear production, no corneal pathologic factors were observed. Further studies are needed to refine this animal model with the ultimate goal of determining optimum delivery route and concentration to reduction in tear production while minimizing side effects in patients.
目的
建立一种动物模型，并研究肉毒毒素 A（BTX-A）局部注射对泪液生成的剂量依赖性影响。
方法
在成人新西兰白兔的泪腺外侧叶中，通过结膜内注射 0.1ml 的 0.625、1.25 或 2.5U BTX-A。在对侧泪腺中，注射 0.1ml 生理盐水。在注射前和注射后 1 周，拍摄照片以评估术前和术后的眼睑位置。使用荧光素和孟加拉玫瑰红染色评估角膜表面，使用 Schirmer 试验评估泪液生成。
结果
与对侧注射生理盐水的腺体相比，注射中间（1.25U）和最高（2.5U）剂量 BTX-A 的腺体在 1 周时泪液生成明显减少（p=0.002 和 0.007）。注射后没有观察到因过度干燥引起的角膜病理因素。虽然观察到注射后出现上睑下垂（p=0.025），但 BTX-A 注射眼与生理盐水注射眼之间没有差异。
结论
在兔子中，BTX-A 局部注射可导致 1 周时泪液生成减少。BTX-A 剂量大于 1.25U 时，泪液生成没有进一步减少，提示在此剂量下腺体受体饱和。尽管抑制了泪液生成，但没有观察到角膜病理因素。需要进一步研究来完善这种动物模型，最终目的是确定最佳的给药途径和浓度，以减少泪液生成，同时最大限度地减少患者的副作用。
Zotero 插件