Department of Chemical Engineering, Izmir Institute of Technology, Gulbahçe Koyu, 35430, Urla, Izmir, Turkey.
J Mater Sci Mater Med. 2013 Feb;24(2):533-46. doi: 10.1007/s10856-012-4804-2.
This study intends to improve blood compatibility of polysulfone (PSF) membranes by generating a nonthrombogenic surface through heparin immobilization. To achieve this task, the support membrane prepared from a blend of PSF and sulfonated polysulfone (SPSF) was modified with layer by layer (LBL) deposition of polyethyleneimine (PEI) and alginate (ALG) and heparin blended with ALG was immobilized only on the outermost surface of the LBL assembly. The results have shown that the adsorption of human plasma proteins and platelet activation on the LBL modified membranes decreased significantly compared with the unmodified PSF and PSF-SPSF blend membranes. Furthermore, blending ALG with a small amount of heparin remarkably prolonged the APTT values of heparin free PEI/ALG coated membranes. It is envisaged that the use of a blend of HEP and ALG only in the terminating layer of the LBL assembly can be an economical and alternative modification technique to create nonthrombogenic surfaces.
本研究旨在通过肝素固定化在聚砜（PSF）膜表面生成抗血栓表面来提高其血液相容性。为实现这一目标，通过层层（LBL）沉积聚乙烯亚胺（PEI）和藻酸钠（ALG）对由 PSF 和磺化聚砜（SPSF）共混物制备的支撑膜进行改性，并且肝素与 ALG 混合仅固定在 LBL 组装体的最外层表面上。结果表明，与未改性的 PSF 和 PSF-SPSF 共混膜相比，LBL 修饰膜上的人血浆蛋白吸附和血小板活化显著降低。此外，少量肝素与 ALG 混合可显著延长无肝素 PEI/ALG 涂层膜的 APTT 值。可以预见，仅在 LBL 组装体的终止层中使用 HEP 和 ALG 的混合物，可以成为一种经济且替代的改性技术，用于创建抗血栓表面。
