Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239 Col. La Escalera Ticomán, 07320 Mexico D.F., Mexico.
Arch Virol. 2013 Mar;158(3):583-99. doi: 10.1007/s00705-012-1525-2. Epub 2012 Nov 6.
Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no information concerning defective dengue virus genomes. Here, we report the detection of defective dengue 2 virus genomes in persistently infected mosquito C6/36 cells. The defective viral genomes were detected at a low ratio compared with the wild-type genome. Deletions of approximately 147 residues (222-368) were found in the E protein, and these mainly affected domain III (73 %) of the protein; deletions of approximately 153 residues (4-156) and 228 residues (597-825) were found in the methyltransferase and polymerase domains, respectively, of the NS5 protein. The truncated versions of NS5 could be detected by western blot only in the protein extracts derived from persistently infected cells.
登革病毒是影响人类的最重要的虫媒病毒，它可以建立持续性感染，特别是在昆虫来源的细胞培养物中。缺陷型病毒基因组与几种黄病毒的持续性感染的建立和维持有关；然而，关于缺陷型登革热病毒基因组的信息几乎不存在。在这里，我们报道了在持续性感染的蚊子 C6/36 细胞中检测到缺陷型登革热 2 型病毒基因组。与野生型基因组相比，缺陷型病毒基因组的检出率较低。在 E 蛋白中发现了大约 147 个残基（222-368）的缺失，这些缺失主要影响该蛋白的结构域 III（73%）；在 NS5 蛋白的甲基转移酶和聚合酶结构域中分别发现了大约 153 个残基（4-156）和 228 个残基（597-825）的缺失。只有在从持续性感染细胞中提取的蛋白质提取物中才能通过 Western blot 检测到截短的 NS5 版本。
