Sheridan Brian S, Lefrançois Leo
University of Connecticut Health Center, Farmington, Connecticut.
Curr Protoc Immunol. 2012 Nov;Chapter 3:3.19.1-3.19.11. doi: 10.1002/0471142735.im0319s99.
The isolation of lymphocytes and other hematopoietic-derived cells from small intestinal tissues has become increasingly relevant to immunology over the last decade. It is also becoming increasingly clear that the impact of local immunity at the mucosal barrier of the intestine has a profound impact on immune responses at distant sites, bringing a new cadre of immunologists to the mucosal frontier. Furthermore, the ability to experimentally manipulate smaller and smaller populations of immune cells has become technologically feasible and in some cases routine. The expanding importance of mucosal immunology coupled with increased technical capabilities requires a standard for experimentally obtaining uniform and consistent cells from the intestinal mucosa. Therefore, it is important to isolate immune cells that are highly viable and minimally manipulated to maximize cellular yields while maintaining acceptable time constraints.
在过去十年中，从小肠组织中分离淋巴细胞和其他造血来源的细胞在免疫学中变得越来越重要。越来越明显的是，肠道黏膜屏障处的局部免疫对远处部位的免疫反应有深远影响，这吸引了一批新的免疫学家投身于黏膜前沿研究。此外，对越来越少数量的免疫细胞进行实验操作在技术上已变得可行，在某些情况下甚至成为常规操作。黏膜免疫学重要性的不断提升以及技术能力的增强，需要一个从肠道黏膜实验获取均匀一致细胞的标准。因此，分离高活性且操作最少的免疫细胞很重要，这样既能在可接受的时间限制内最大化细胞产量。
