Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Pakistan.
Prostate. 2013 May;73(7):690-9. doi: 10.1002/pros.22609. Epub 2012 Nov 5.
Kisspeptin peptides mediate their actions through the GnRH loop system. How kisspeptins affect prostate gland in prepubertal male mammals remains elusive.
To address this kisspeptin was administered as subchronic (12 days) twice daily i.p. dose at three different dosage regimens: 10 pg, 1 ng and 1 µg, to prepubertal male Sprague-Dawley rats (PND 35). Control rats were maintained in parallel. At the end of the experiment prostate gland was dissected out and processed for light and electron microscopy. DNA damage was also estimated by DNA ladder assay and DNA fragmentation assay.
Prostate weights decreased significantly (P < 0.05) at 1 µg treatment dose of kisspeptin. The epithelial height of secretory acini of prostate decreased at 10 pg (P < 0.05), 1 ng, and 1 µg doses (P < 0.001). Histomorphology and ultrastructure demonstrated, decrease in epithelial cell height, epithelial folding and dilatation of the organelles with kisspeptin treatment. Percent DNA damage to the prostatic tissue was 20.74 ± 2.18, 43.60 ± 2.39, and 58.18 ± 2.59 at 10 pg, 1 ng and 1 µg doses, respectively.
The study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of prepubertal prostate gland. Wiley Periodicals, Inc.
Kisspeptin 肽通过 GnRH 环系统发挥其作用。Kisspeptin 如何影响青春期前雄性哺乳动物的前列腺仍不清楚。
为了解决这个问题，将 Kisspeptin 作为亚慢性（12 天）每天两次腹腔内给药，剂量分为三种不同的方案：10pg、1ng 和 1μg，给予青春期前的雄性 Sprague-Dawley 大鼠（PND35）。对照大鼠在平行条件下饲养。实验结束时，取出前列腺并进行光镜和电镜检查。还通过 DNA 梯状电泳和 DNA 片段化分析来估计 DNA 损伤。
Kisspeptin 的 1μg 治疗剂量显著降低了前列腺的重量（P < 0.05）。前列腺分泌腺的上皮高度在 10pg（P < 0.05）、1ng 和 1μg 剂量下降低（P < 0.001）。组织形态学和超微结构显示，随着 Kisspeptin 的处理，上皮细胞高度、上皮折叠和细胞器扩张减少。前列腺组织的 DNA 损伤百分比分别为 20.74±2.18、43.60±2.39 和 58.18±2.59。
该研究表明，连续给予 Kisspeptin 不会导致青春期前前列腺早期成熟，而是导致严重退化。Wiley Periodicals, Inc.
