Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan 29050, Pakistan.
Reprod Biol Endocrinol. 2012 Mar 10;10:18. doi: 10.1186/1477-7827-10-18.
Degenerative effects of critical regulators of reproduction, the kisspeptin peptides, on cellular aspects of sexually immature male gonads are known but similar information on accessory sex glands remain elusive.
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day. Control rats were maintained in parallel. The day following the end of the experimental period, seminal vesicles were removed and processed for light and electron microscopic examination using the standard methods. DNA damage was estimated by DNA ladder assay and DNA fragmentation assay.
The results demonstrated cellular degeneration. Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05). Marked decrease in epithelial folds was readily noticeable, while the lumen was dilated. Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules. Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.
The study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of sexually immature seminal vesicles.
已知，关键性生殖调节因子 kisspeptin 肽对未成熟雄性性腺的细胞方面具有退行性影响，但类似的信息对附属性生殖腺仍然难以捉摸。
对未成熟的实验室大鼠以三种不同的剂量浓度（10pg、1ng 和 1μg）连续 12 天每天两次注射 kisspeptin-10。平行对照维持不变。实验结束后的第二天，取出精囊并采用标准方法进行光镜和电镜检查。通过 DNA 梯状电泳和 DNA 片段化分析来估计 DNA 损伤。
结果显示细胞发生了退行性变化。所有剂量组（P<0.05）的精囊上皮细胞高度均显著降低。上皮皱襞明显减少，同时管腔扩张。超微结构变化的特征是内质网和高尔基体扩张、核异染色质、核膜内陷和分泌颗粒数量减少。10pg、1ng 和 1μg 剂量组的精囊 DNA 损伤百分比分别为 19.54±1.98%、38.06±2.09%和 58.18±2.59%。
该研究表明，连续给予 kisspeptin 不会导致未成熟精囊的早期成熟，而是导致严重的退行性变化。
