Cabré F, Cascante M, Canela E I
Department of Biochemistry and Physiology, Faculty of Chemistry, University of Barcelona, Spain.
J Biochem Biophys Methods. 1990 Jan;20(2):123-35. doi: 10.1016/0165-022x(90)90071-j.
The aim of this paper is to apply inverse regression as a method for treating experimental data obtained from gel filtration chromatography in order to obtain estimates of hydrodynamic parameters of globular proteins with true confidence intervals. The method is illustrated with the determination, using inverse regression, of molecular mass and Stokes radius for four test proteins (aldolase, chymotrypsinogen A, aldehyde oxidase and xanthine oxidase), from experimental data obtained with a Sephacryl S-300 column. A simple personal computer (PC) program written in standard basic, that is useful for this purpose, is included.
本文的目的是应用逆回归作为一种处理从凝胶过滤色谱法获得的实验数据的方法，以便获得具有真实置信区间的球状蛋白质流体动力学参数的估计值。使用逆回归从用Sephacryl S - 300柱获得的实验数据中测定四种测试蛋白质（醛缩酶、胰凝乳蛋白酶原A、醛氧化酶和黄嘌呤氧化酶）的分子量和斯托克斯半径来说明该方法。文中包含了一个用标准Basic语言编写的、用于此目的的简单个人计算机（PC）程序。
