Cabré F, Canela E I, Canela M A
Department of Biochemistry and Physiology, University of Barcelona, Catalonia, Spain.
J Chromatogr. 1989 Jun 23;472(2):347-56. doi: 10.1016/s0021-9673(00)94133-5.
The accuracy and precision of the estimates of hydrodynamic parameters of globular proteins obtained by inverse regression from gel filtration chromatographic data are discussed. The usefulness of gel filtration chromatography as the basis for a rapid and reliable method for the determination of the Stokes radius and the molecular mass is considered. The discussion is supported by an analysis of the models already proposed in the literature, and is based on the precision of the estimates.
讨论了通过凝胶过滤色谱数据进行逆回归获得的球状蛋白质流体动力学参数估计值的准确性和精确性。考虑了凝胶过滤色谱作为一种快速可靠的测定斯托克斯半径和分子量方法基础的实用性。通过对文献中已提出的模型进行分析，并基于估计值的精确性来支持该讨论。
