Kikuchi Y, Miyauchi M, Nagata I
Department of Obstetrics and Gynecology, National Defence Medical College, Saitama.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Feb;42(2):121-8.
In vitro and in vivo effects of prostaglandin D2 on human ovarian cancer growth were examined by using a cell line, designated HR, derived from ascites of patient with serous cystadenocarcinoma of the ovary. The HR cell proliferation in vitro was dose-dependently inhibited at prostaglandin D2 concentrations between 0.1 and 4.0 micrograms per ml. The results of a 51Cr-release assay seemed to indicate that the inhibition resulted from a direct cytotoxic effect exerted by prostaglandin D2. All DNA, RNA, and protein synthesis by the HR cells was also inhibited in a dose-dependent manner with 48 hr exposure to prostaglandin D2. When nude mice were inoculated with 5 X 10(5) HR cells, the 50% survival time in the untreated group was 52 days. The 50% survival time of nude mice treated with 12 mg (but not 4 mg) of prostaglandin D2 per kg was significantly prolonged to 67 days, in addition to a significant inhibition of the tumor growth. Adjuvant effects of prostaglandin D2 on cisplatin in relation to tumor growth were also studied. Combinations of 0.2 or 0.4 microgram cisplatin per ml and 0.05 or 0.1 microgram prostaglandin D2 per ml, which did not affect the HR cell proliferation alone, resulted in a significant inhibition of cell proliferation. In addition, the tumor take of HR cells by nude mice in groups treated with a combination of cisplatin and prostaglandin D2 was inhibited.(ABSTRACT TRUNCATED AT 250 WORDS)
利用一种名为HR的细胞系，该细胞系源自一名卵巢浆液性囊腺癌患者的腹水，研究了前列腺素D2对人卵巢癌生长的体内外作用。在每毫升0.1至4.0微克的前列腺素D2浓度下，HR细胞在体外的增殖受到剂量依赖性抑制。51铬释放试验的结果似乎表明，这种抑制是由前列腺素D2直接产生的细胞毒性作用所致。HR细胞的所有DNA、RNA和蛋白质合成在暴露于前列腺素D2 48小时后也受到剂量依赖性抑制。当给裸鼠接种5×10(5)个HR细胞时，未治疗组的50%存活时间为52天。每千克用12毫克（而非4毫克）前列腺素D2治疗的裸鼠的50%存活时间显著延长至67天，同时肿瘤生长也受到显著抑制。还研究了前列腺素D2对顺铂在肿瘤生长方面的辅助作用。每毫升0.2或0.4微克顺铂与每毫升0.05或0.1微克前列腺素D2的组合，单独使用时不影响HR细胞增殖，但却导致细胞增殖受到显著抑制。此外，顺铂和前列腺素D2联合治疗组的裸鼠对HR细胞的肿瘤接种也受到抑制。（摘要截短于250字）
