Fuchtner C, Emma D A, Manetta A, Gamboa G, Bernstein R, Liao S Y
Department of Obstetrics and Gynecology, University of California, Orange 92668.
Gynecol Oncol. 1993 Feb;48(2):203-9. doi: 10.1006/gyno.1993.1034.
A new epithelial ovarian carcinoma cell line (UCI 101) has been established from the ascitic fluids and solid tumor of a patient with progressive papillary adenocarcinoma of the ovary shown previously to be refractory to combination chemotherapy consisting of cyclophosphamide, Adriamycin, and cisplatin as well as single-agent chemotherapy of taxol and high-dose cisplatin. The UCI 101 cell line grows well with an in vitro doubling time of 24 hr. The cell line expresses the B 72.3 (Tag 72), CA125, MH99 (ESA), and E29 (EMA) cell surface antigens and AE1/AE3 cytokeratins. This cell line overexpresses (as determined by immunocytochemistry) both p-glycoprotein and the epidermal growth factor receptor. The in vitro drug response to single agents including Adriamycin, cisplatin, dequalinium chloride, etoposide, 5-fluorouracil, taxol, and tumor necrosis factor was examined. Intraperitoneal transplantation of the cells into athymic mice resulted in foci of tumor on all peritoneal surfaces including the viscera and diaphragm ultimately leading to solid bulky disease with massive production of ascites. High levels of CA125 (> 500 units/ml) were detected in the serum of tumor-bearing mice. Cytogenetic analysis of cultured cells shows several marker chromosomes containing deletions, duplications, and translocations. Cytologic and histologic evaluation of the xenograft revealed morphological characteristics identical to those of the original tumor.
一种新的上皮性卵巢癌细胞系（UCI 101）已从一名卵巢乳头状腺癌患者的腹水和实体瘤中建立，该患者先前对由环磷酰胺、阿霉素和顺铂组成的联合化疗以及紫杉醇和高剂量顺铂的单药化疗均耐药。UCI 101细胞系生长良好，体外倍增时间为24小时。该细胞系表达B 72.3（Tag 72）、CA125、MH99（ESA）和E29（EMA）细胞表面抗原以及AE1/AE3细胞角蛋白。该细胞系（通过免疫细胞化学测定）过表达P-糖蛋白和表皮生长因子受体。检测了该细胞系对包括阿霉素、顺铂、氯化喹吖因、依托泊苷、5-氟尿嘧啶、紫杉醇和肿瘤坏死因子在内的单药的体外药物反应。将细胞腹腔内移植到无胸腺小鼠体内，导致包括内脏和膈肌在内的所有腹膜表面出现肿瘤灶，最终导致形成大量腹水的实性肿块性疾病。在荷瘤小鼠血清中检测到高水平的CA125（>500单位/毫升）。对培养细胞的细胞遗传学分析显示几条标记染色体存在缺失、重复和易位。对异种移植瘤的细胞学和组织学评估显示其形态特征与原发肿瘤相同。
