College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China.
Immunogenetics. 2013 Feb;65(2):125-32. doi: 10.1007/s00251-012-0662-9. Epub 2012 Nov 7.
The Toll-like receptor 5 (TLR5) plays a crucial role in host defense against flagellated bacteria by recognizing flagellin. Accumulating evidence suggests that single nucleotide polymorphisms (SNPs) in TLR5 have an effect on flagellin recognition and are associated with susceptibility/resistance to disease. In this study, we analyzed association of SNPs, including c.834T>G, c.1065T>C, c.1205C>T, c.1246A>T, c.1269G>A, and c.1398C>T, as well as mRNA level of TLR5 with the abundance of transcripts of cytokines in pigs. SNPs c.1246A>T and c.1269G>A were significantly associated with the transcript abundance of interleukin (IL)-2, and SNPs c.834T>G and c.1398C>T with IL-10 (P < 0.05); the haplotypes showed a tendency to affect the transcript abundance of IL-10 (P = 0.0660) and significantly associated with the transcription of TLR5 (P < 0.01); the abundance of transcripts of TLR5 and IL-10 were strongly correlated (P < 0.01). The results indicated that the SNPs, associated with the transcript abundance of cytokines, were related to immune responsiveness mediated by cytokine, which, in turn, would have a role in pig breeding for disease resistance. Furthermore, the positive correlation between the abundance of TLR5 and IL10 suggest a link between TLR5 activation and IL-10 expression in porcine.
Toll 样受体 5（TLR5）通过识别鞭毛蛋白在宿主防御鞭毛细菌中发挥关键作用。越来越多的证据表明，TLR5 中的单核苷酸多态性（SNP）对鞭毛蛋白识别有影响，并与疾病的易感性/抗性有关。在这项研究中，我们分析了包括 c.834T>G、c.1065T>C、c.1205C>T、c.1246A>T、c.1269G>A 和 c.1398C>T 在内的 SNP 以及 TLR5 的 mRNA 水平与猪细胞因子转录物丰度的相关性。SNP c.1246A>T 和 c.1269G>A 与白细胞介素（IL）-2 的转录物丰度显著相关，SNP c.834T>G 和 c.1398C>T 与 IL-10 相关（P<0.05）；单倍型显示出影响 IL-10 转录物丰度的趋势（P=0.0660），并与 TLR5 的转录显著相关（P<0.01）；TLR5 和 IL-10 的转录物丰度呈强相关性（P<0.01）。结果表明，与细胞因子转录物丰度相关的 SNP 与细胞因子介导的免疫反应性有关，这反过来又在猪的疾病抗性育种中发挥作用。此外，TLR5 和 IL10 的丰度之间的正相关表明 TLR5 激活与猪中 IL-10 表达之间存在联系。
