Keirstead N D, Hayes M A, Vandervoort G E, Brooks A S, Squires E J, Lillie B N
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Vet Immunol Immunopathol. 2011 Jul 15;142(1-2):1-13. doi: 10.1016/j.vetimm.2011.02.011. Epub 2011 Feb 25.
Innate immune recognition of pathogens involves various surface receptors and soluble proteins that precede agglutination, complement activation, phagocytosis, and the adaptive immune response. Mannan-binding lectins (MBLs), ficolins (FCNs) and surfactant protein A (SP-A) are soluble collagenous lectins that bind surface structures of various bacteria, viruses and fungi. Some single nucleotide polymorphisms (SNPs) in collagenous lectin genes of humans and other species, including pigs, have been implicated in variation in susceptibility to infectious and inflammatory diseases. In this study we determined the frequencies of 13 SNP alleles of MBL-A, MBL-C, ficolin-α, ficolin-β, and SP-A in 1324 healthy pigs and 461 pigs diagnosed with common infectious diseases at necropsy. For comparison, we also analyzed 12 other SNP alleles in several other innate immune genes, including galectins and TLRs. Several SNPs within genes encoding porcine MBL-A, MBL-C and SP-A were more frequent in pigs diagnosed at necropsy with various diseases or pathogens. These findings suggest that several collagenous lectin SNPs are associated with disease susceptibility and therefore might be genetic markers of impaired innate immune function.
对病原体的天然免疫识别涉及各种表面受体和可溶性蛋白，这些在凝集、补体激活、吞噬作用和适应性免疫反应之前就已存在。甘露聚糖结合凝集素（MBL）、纤维胶凝蛋白（FCN）和表面活性蛋白A（SP-A）是可溶性胶原凝集素，它们能结合各种细菌、病毒和真菌的表面结构。人类和其他物种（包括猪）的胶原凝集素基因中的一些单核苷酸多态性（SNP）与感染性和炎性疾病易感性的差异有关。在本研究中，我们测定了1324头健康猪和461头在尸检时被诊断患有常见传染病的猪中MBL-A、MBL-C、纤维胶凝蛋白-α、纤维胶凝蛋白-β和SP-A的13个SNP等位基因的频率。为作比较，我们还分析了其他几个天然免疫基因（包括半乳糖凝集素和Toll样受体）中的12个其他SNP等位基因。在编码猪MBL-A、MBL-C和SP-A的基因中的几个SNP在尸检时被诊断患有各种疾病或感染病原体的猪中更为常见。这些发现表明，几个胶原凝集素SNP与疾病易感性相关，因此可能是天然免疫功能受损的遗传标记。
