Sherchan J B, Ohara H, Sakurada S, Basnet A, Tandukar S, Sherchand J B, Bam D S
Kathmandu University School of Medical Sciences, Dhulikhel, Nepal.
Kathmandu Univ Med J (KUMJ). 2012 Apr-Jun;10(38):14-7. doi: 10.3126/kumj.v10i2.7336.
Enteric opportunistic parasitic infections are the major source of diarrheal disease in developing countries mainly in Human Immunodeficiency virus (HIV) infected patients.
The study was to detect enteric parasites causing diarrhea and their association with immune status in HIV-seropositive patients.
The present study was conducted in Dirgh-Jeevan Health Care Research Center and Tribhuvan University Teaching Hospital, Public Health Research Laboratory, Kathmandu, Nepal between June 2010 and May 2011 involving 146 Human Immunodeficiency virus (HIV) positive patients. Serostatus from these patients were detected by Enzyme Linked Immunosorbent assay. CD4+ T cell counts were done by flow cytometry. Stool was examined for enteric parasites by microscopy with special staining methods.
A total of 146 HIV sero-positive patients with and without diarrhea age between 20 to 45 years were included in the study. Of the 146 patients, the protozoan parasitic infection was found in 30.13% (44/146). Out of 146 patients, 78 had diarrhea in which parasitic infection was 39 (50%) and 7.35% (5/68) protozoal parasites positive cases did not have diarrhea. A significant difference (p less than 0.05) was observed in the level of infection of intestinal protozoan between the HIV seropositive with diarrhea and HIV-seropositive without diarrhea. Out of 43 patients whose CD4+ T cells were less than 200/μl, 29 (67.4%) had opportunistic parasitic infection whereas out of 103 patients whose CD4+ T cells were =200/mcl, only 15 (14.56%) had opportunistic parasitic infection (P less than 0.05).
Enteric opportunistic parasitic infections were detected in 30.1% among HIV-seropositive patients and low CD4+ T count indicated high enteric opportunistic infection. Early detection of enteric parasitic infections will help in the management and to improve the quality of life for HIV-infected individuals.
肠道机会性寄生虫感染是发展中国家腹泻病的主要来源，主要发生在感染人类免疫缺陷病毒（HIV）的患者中。
本研究旨在检测导致腹泻的肠道寄生虫及其与HIV血清阳性患者免疫状态的关联。
本研究于2010年6月至2011年5月在尼泊尔加德满都的迪尔格 - 吉万医疗保健研究中心、特里布万大学教学医院公共卫生研究实验室进行，涉及146例人类免疫缺陷病毒（HIV）阳性患者。通过酶联免疫吸附测定法检测这些患者的血清状态。通过流式细胞术进行CD4 + T细胞计数。采用特殊染色方法通过显微镜检查粪便中的肠道寄生虫。
本研究共纳入146例年龄在20至45岁之间、有或无腹泻的HIV血清阳性患者。在146例患者中，原生动物寄生虫感染率为30.13%（44/146）。146例患者中，78例有腹泻，其中寄生虫感染39例（50%），7.35%（5/68）原生动物寄生虫阳性病例无腹泻。HIV血清阳性且有腹泻患者与HIV血清阳性且无腹泻患者的肠道原生动物感染水平存在显著差异（p小于0.05）。在43例CD4 + T细胞小于200/μl的患者中，29例（67.4%）有机会性寄生虫感染，而在103例CD4 + T细胞=200/μl的患者中，只有15例（14.56%）有机会性寄生虫感染（P小于0.05）。
在HIV血清阳性患者中，肠道机会性寄生虫感染率为30.1%，低CD4 + T细胞计数表明肠道机会性感染率高。早期检测肠道寄生虫感染将有助于管理并改善HIV感染者的生活质量。
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