CASA 检测到的人类精子异常：与染色质包装和 DNA 碎片化的相关性。
CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation.
机构信息
Genetics Department, Krishna IVF Clinic, Krishna Nagar, Visakhapatnam, 530 002, Andhra Pradesh, India.
出版信息
J Assist Reprod Genet. 2012 Dec;29(12):1327-34. doi: 10.1007/s10815-012-9885-9. Epub 2012 Nov 8.
PURPOSE
The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI.
METHODS
Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively.
RESULTS
Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal.
CONCLUSIONS
Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.
目的
本研究旨在评估人类精子形态动力学异常对染色质包装和 DNA 完整性的影响，以及精子选择在 ICSI 中的可能有益作用。
方法
使用 CASA 分析了 1002 名患者的精液样本的形态和活力。通过吖啶橙染色分析鱼精蛋白状态，通过精子染色质扩散试验分析 DNA 碎片化。
结果
在畸形精子症和少弱畸形精子症组中，细长形、圆形、pyriform、不定形、微小和巨大形态的精子明显更高。与正常组相比，这些异常组的染色质包装和 DNA 碎片化指数存在显著差异。同样，在弱精子症组中，异常运动参数与 DNA 碎片化指数之间也存在显著相关性。
结论
特定的异常形态形式具有更高的染色质包装异常和 DNA 碎片化发生率。在 ICSI 中使用这些精子可能会对受精、胚胎发育和流产率产生影响。在 ICSI 过程中可以选择性地避免这些精子，以改善 ART 结果。
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