Sivanarayana T, Ravi Krishna Ch, Jaya Prakash G, Krishna K M, Madan K, Sudhakar G, Rama Raju G A
Genetics Department Krishna IVF Clinic Krishna Nagar 530 002 Visakhapatnam Andhra Pradesh India.
Department of Human Genetics Andhra University 530 003 Visakhapatnam Andhra Pradesh India.
Reprod Med Biol. 2013 Sep 1;13(2):87-94. doi: 10.1007/s12522-013-0168-7. eCollection 2014 Apr.
The aim of the present study was to investigate the relationship between sperm DNA fragmentation index (sDFI) and outcome of intracytoplasmic sperm injection (ICSI).
All the patients were divided into two groups based on sperm DNA fragmentation analysis by the sperm chromatin dispersion (SCD) method. A total of 237 patients were in the DNA fragmentation normal group (sDFI ≤ 30 %), and 140 patients were in the DNA fragmentation abnormal group (sDFI ≥ 30 %). The relationship of sDFI with the outcome of ICSI was analyzed.
A significant difference in semen parameters was observed between the DNA fragmentation normal and abnormal groups [count, motility and morphology ( < 0.05)]. However, no significant difference was seen between the number of oocytes retrieved and fertilization rates between the two groups, whereas the number of embryos progressed to day 3 and the blastocyst formation rate in the remaining embryos after transfer were significantly more in the DNA fragmentation normal group ( < 0.05). A significant negative correlation was noted between DFI values of more than 30 % and number of pregnancies and deliveries ( < 0.05). A higher DFI was also associated with increased abortion rates.
In the present study, sperm with DNA fragmentation showed a negative correlation with semen parameters. Further, sperm with damaged DNA have potential adverse effects on embryo progression, clinical pregnancy rate, and ongoing pregnancies.
本研究旨在探讨精子DNA碎片指数（sDFI）与卵胞浆内单精子注射（ICSI）结局之间的关系。
通过精子染色质扩散（SCD）法对精子DNA碎片进行分析，将所有患者分为两组。DNA碎片正常组（sDFI≤30%）共有237例患者，DNA碎片异常组（sDFI≥30%）有140例患者。分析sDFI与ICSI结局的关系。
DNA碎片正常组与异常组之间精液参数存在显著差异[计数、活力和形态（<0.05）]。然而，两组之间回收的卵母细胞数量和受精率没有显著差异，而DNA碎片正常组中发育到第3天的胚胎数量以及移植后剩余胚胎的囊胚形成率显著更高（<0.05）。DFI值超过30%与妊娠和分娩数量之间存在显著负相关（<0.05）。较高的DFI也与流产率增加有关。
在本研究中，具有DNA碎片的精子与精液参数呈负相关。此外，DNA受损的精子对胚胎发育、临床妊娠率和持续妊娠具有潜在的不利影响。
