Karydis Spyros, Asimakopoulos Byron, Papadopoulos Nikos, Vakalopoulos Ioannis, Al-Hasani Safaa, Nikolettos Nikos
Laboratory of Physiology, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Greece.
In Vivo. 2005 Sep-Oct;19(5):921-5.
The condensation of sperm chromatin during spermiogenesis and epididymal transport is of essential importance for fertilization. The main purpose of this study was to examine whether abnormalities of sperm nuclear condensation can influence the outcome of intracytoplasmic sperm injection (ICSI) cycles.
Semen samples from 154 ICSI cycles were studied. Before semen preparation for ICSI, basic semen analysis was performed and a small portion from each sample was fixed. The condensation of sperm nuclear chromatin was evaluated with chromomycin A3 under a fluorescence microscope.
The incidence of spermatozoa with abnormal chromatin condensation was positively correlated with sperm concentration (p = 0.020565), but was not correlated with other semen parameters such as morphology and motility. Abnormal chromatin condensation was also not correlated with fertilization rate, cumulative embryo score or pregnancy rate.
The above results indicate that ICSI outcome is not influenced by the incidence of spermatozoa with abnormal chromatin condensation.
精子发生和附睾运输过程中精子染色质的凝聚对于受精至关重要。本研究的主要目的是检验精子核凝聚异常是否会影响卵胞浆内单精子注射（ICSI）周期的结果。
对154个ICSI周期的精液样本进行研究。在为ICSI准备精液之前，进行基本精液分析，并从每个样本中取一小部分进行固定。在荧光显微镜下用放线菌素A3评估精子核染色质的凝聚情况。
染色质凝聚异常的精子发生率与精子浓度呈正相关（p = 0.020565），但与其他精液参数如形态和活力无关。染色质凝聚异常也与受精率、累积胚胎评分或妊娠率无关。
上述结果表明，染色质凝聚异常的精子发生率不影响ICSI结果。
