Amin Huma, Mohsin Shahida, Aslam Maria, Hussain Shabbir, Saeed Tahir, Ullah M Ikram, Sami Waqas
Department of Haematology, University of Health Sciences, Lahore, Pakistan.
Blood Coagul Fibrinolysis. 2012 Dec;23(8):745-50. doi: 10.1097/MBC.0b013e328358e913.
Aging is associated with increased levels of coagulation factors and decrease in natural anticoagulant factors. This strongly supports that age-related hypercoagulable state occurs in elderly. This study aimed to measure the plasma levels of coagulation factors and anticoagulant levels in young and elderly to observe the effect of age on haemostatic system. Ninety healthy individuals, both men and women were divided into two groups on the basis of age. Group I included participants of less than 40 years of age, whereas, group II comprised of participants more than 60 years of age. Fibrinogen activity was assessed by using Clauss technique. Coagulation factor VII, and factor VIII activity by corresponding one stage assay based on prothrombin time and activated partial thromboplastin time. Antithrombin III was measured by the chromogenic method. Our results showed that significantly increased levels of fibrinogen (P = 0.001) were observed in the elderly population as compared to young. Higher fibrinogen levels in younger women than men and comparatively higher level than other races was observed in our local population. Increase in factor VII levels (P = 0.05) was also observed in the elderly group. This increase was statistically significant with age in women (P = 0.03). Factor VIII rose with age in both sexes (P = 0.001). Higher antithrombin activity was observed in the younger group whereas the older group demonstrated significantly lower antithrombin activity (P = 0.001). We conclude that considerable effects of age and sex are observed on coagulation factors and naturally occurring inhibitors.
衰老与凝血因子水平升高及天然抗凝因子减少有关。这有力地支持了老年人会出现与年龄相关的高凝状态。本研究旨在测量年轻人和老年人血浆中凝血因子水平及抗凝水平，以观察年龄对止血系统的影响。90名健康个体，包括男性和女性，根据年龄分为两组。第一组包括年龄小于40岁的参与者，而第二组由年龄大于60岁的参与者组成。采用克劳斯技术评估纤维蛋白原活性。根据凝血酶原时间和活化部分凝血活酶时间，通过相应的一步法测定凝血因子VII和因子VIII活性。采用发色底物法测定抗凝血酶III。我们的结果显示，与年轻人相比，老年人群中纤维蛋白原水平显著升高（P = 0.001）。在我们的本地人群中，观察到年轻女性的纤维蛋白原水平高于男性，且相对高于其他种族。老年组中也观察到因子VII水平升高（P = 0.05）。在女性中，这种升高与年龄具有统计学意义（P = 0.03）。因子VIII在两性中均随年龄升高（P = 0.001）。在较年轻组中观察到较高的抗凝血酶活性，而老年组的抗凝血酶活性显著较低（P = 0.001）。我们得出结论，年龄和性别对凝血因子及天然存在的抑制剂有显著影响。
