Department of Biological Sciences, Western Illinois University, Macomb, IL 61455, USA.
J Chem Ecol. 2012 Oct;38(10):1247-63. doi: 10.1007/s10886-012-0205-8. Epub 2012 Nov 8.
Microarray analysis was used to measure the impact of herbivory by Helicoverpa zea, (corn earworm caterpillar) on wild-type and transgenic tomato, Solanum lycopersicum, plants that over-express peroxidase. Caterpillar herbivory had by far the greatest affect on gene expression, but the peroxidase transgene also altered the expression of a substantial number of tomato genes. Particularly high peroxidase activity resulted in the up-regulation of genes encoding proteinase inhibitors, pathogenesis-related (PR) proteins, as well as proteins associated with iron and calcium transport, and flowering. In a separate experiment conducted under similar conditions, real-time quantitative polymerase chain reaction (qPCR) analysis confirmed our microarray results for many genes. There was some indication that multiple regulatory interactions occurred due to the interaction of the different treatments. While herbivory had the greatest impact on tomato gene expression, our results suggest that levels of expression of a multifunctional gene, such as peroxidase and its products, can influence other gene expression systems distinct from conventional signaling pathways, further indicating the complexity of plant defensive responses to insects.
利用微阵列分析技术，我们测量了玉米穗虫（Helicoverpa zea）对野生型和过表达过氧化物酶的转基因番茄（Solanum lycopersicum）植株的取食影响。迄今为止，毛毛虫的取食对基因表达的影响最大，但过氧化物酶转基因也改变了大量番茄基因的表达。特别高的过氧化物酶活性导致蛋白酶抑制剂、与发病相关（PR）蛋白以及与铁和钙运输和开花相关的蛋白的基因上调。在类似条件下进行的另一个实验中，实时定量聚合酶链反应（qPCR）分析证实了我们对许多基因的微阵列结果。有迹象表明，由于不同处理的相互作用，发生了多种调节相互作用。虽然取食对番茄基因表达的影响最大，但我们的结果表明，多功能基因（如过氧化物酶及其产物）的表达水平可以影响不同于传统信号通路的其他基因表达系统，这进一步表明了植物对昆虫防御反应的复杂性。
