Research Faculty of Agriculture, Hokkaido University , Kita 9 Nishi 9, Kita, Sapporo, Hokkaido 060-8589, Japan.
Breed Sci. 2012 Jan;61(5):480-94. doi: 10.1270/jsbbs.61.480. Epub 2012 Feb 4.
Herbicide-resistant transgenic soybean plants hold a leading market share in the USA and other countries, but soybean has been regarded as recalcitrant to transformation for many years. The cumulative and, at times, exponential advances in genetic manipulation have made possible further choices for soybean transformation. The most widely and routinely used transformation systems are cotyledonary node-Agrobacterium-mediated transformation and somatic embryo-particle-bombardment-mediated transformation. These ready systems enable us to improve seed qualities and agronomic characteristics by transgenic approaches. In addition, with the accumulation of soybean genomic resources, convenient or promising approaches will be requisite for the determination and use of gene function in soybean. In this article, we describe recent advances in and problems of soybean transformation, and survey the current transgenic approaches for applied and basic research in Japan.
抗除草剂转基因大豆植物在美国和其他国家占据着主导市场份额,但多年来,大豆一直被认为是转化的顽疾。遗传操作的累积和有时呈指数级的进步,为大豆的转化提供了更多的选择。最广泛和常规使用的转化系统是子叶节 - 农杆菌介导的转化和体细胞胚 - 粒子轰击介导的转化。这些成熟的系统使我们能够通过转基因方法改善种子质量和农艺特性。此外,随着大豆基因组资源的积累,确定和利用大豆基因功能的便捷或有前途的方法将是必要的。在本文中,我们描述了大豆转化的最新进展和问题,并调查了日本应用和基础研究中当前的转基因方法。