Donaldson P A, Simmonds D H
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1-613-7596566 e-mail:
Plant Cell Rep. 2000 Apr;19(5):478-484. doi: 10.1007/s002990050759.
Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and β-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27-92%, but transformation events were usually restricted to non-regenerable callus.
短季适应型大豆[Glycine max (L.) Merrill]基因型（成熟组0和00）在与根癌农杆菌菌株A281、C58和ACH5进行的肿瘤形成试验中对根癌农杆菌敏感。其反应取决于细菌菌株和植物品种。用根癌农杆菌EHA105/pBI121对这些基因型的子叶节外植体进行体外农杆菌介导的转化效率低下，但获得了一株携带新霉素磷酸转移酶和β-葡萄糖醛酸酶（gus）转基因连锁插入的转基因AC Colibri植株。转基因被传递给后代，并且在T代中检测到稳定的gus表达。从共培养的子叶外植体中获得转基因植株的低回收率归因于可再生细胞的转化效率低下，和/或此类细胞的选择或存活不佳，而不是对农杆菌的易感性差，因为根据品种不同，外植体的转化率为27%-92%，但转化事件通常局限于不可再生的愈伤组织。
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