Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori 680-0001, Japan.
Breed Sci. 2012 Mar;62(1):38-45. doi: 10.1270/jsbbs.62.38. Epub 2012 Mar 20.
Aegilops tauschii Coss. is the D-genome donor to hexaploid bread wheat (Triticum aestivum) and is the most promising wild species as a genetic resource for wheat breeding. To study the population structure and diversity of 81 Ae. tauschii accessions collected from various regions of its geographical distribution, the genomic representation of these lines were used to develop a diversity array technology (DArT) marker array. This Ae. tauschii array and a previously developed DArT wheat array were used to scan the genomes of the 81 accessions. Out of 7500 markers (5500 wheat and 2000 Ae. tauschii), 4449 were polymorphic (3776 wheat and 673 Ae. tauschii). Phylogenetic and population structure studies revealed that the accessions could be divided into three groups. The two Ae. tauschii subspecies could also be separately clustered, suggesting that the current taxonomy might be valid. DArT markers are effective to detect very small polymorphisms. The information obtained about Ae. tauschii in the current study could be useful for wheat breeding. In addition, the new DArT array from this Ae. tauschii population is expected to be an effective tool for hexaploid wheat studies.
节节麦 Coss. 是六倍体普通小麦（Triticum aestivum）的 D 基因组供体，是小麦育种中最有前途的野生物种遗传资源。为了研究 81 份来自节节麦地理分布不同地区的群体结构和多样性，利用这些系的基因组代表开发了多样性阵列技术（DArT）标记阵列。该节节麦阵列和以前开发的 DArT 小麦阵列用于扫描 81 个品系的基因组。在 7500 个标记（5500 个小麦和 2000 个节节麦）中，有 4449 个多态性（3776 个小麦和 673 个节节麦）。系统发育和群体结构研究表明，这些品系可以分为三组。两个节节麦亚种也可以分别聚类，这表明当前的分类可能是有效的。DArT 标记有效地检测非常小的多态性。本研究中获得的关于节节麦的信息对小麦育种可能有用。此外，来自该节节麦群体的新 DArT 阵列有望成为六倍体小麦研究的有效工具。
