Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, Alberta, Canada.
Theor Appl Genet. 2011 May;122(8):1547-60. doi: 10.1007/s00122-011-1554-3. Epub 2011 Mar 11.
Triticale (X Triticosecale Wittm.) is a hybrid derived by crossing wheat (Triticum sp.) and rye (Secale sp.). Till date, only a limited number of simple sequence repeat (SSRs) markers have been used in triticale molecular analyses and there is a need to identify dedicated high-throughput molecular markers to better exploit this crop. The objective of this study was to develop and evaluate diversity arrays technology (DArT) markers in triticale. DArT marker technology offers a high level of multiplexing. Development of new markers from triticale accessions was combined with mining the large collection of previously developed markers in rye and wheat. Three genotyping arrays were used to analyze a collection of 144 triticale accessions. The polymorphism level ranged from 8.6 to 23.8% for wheat and rye DArT markers, respectively. Among the polymorphic markers, rye markers were the most abundant (3,109) followed by wheat (2,214) and triticale (719). The mean polymorphism information content values were 0.34 for rye DArT markers and 0.37 for those from triticale and wheat. High correlation was observed between similarity matrices derived from rye, triticale, wheat and combined marker sets, as well as for the cophenetic values matrices. Cluster analysis revealed genetic relationships among the accessions consistent with the agronomic and pedigree information available. The newly developed triticale DArT markers as well as those originated from rye and wheat provide high quality markers that can be used for diversity analyses and might be exploited in a range of molecular breeding and genomics applications in triticale.
小黑麦（X Triticosecale Wittm.）是通过小麦（Triticum sp.）和黑麦（Secale sp.）杂交形成的杂种。迄今为止，仅使用了有限数量的简单序列重复（SSR）标记进行小黑麦分子分析，因此需要鉴定专用的高通量分子标记，以更好地利用该作物。本研究的目的是开发和评估多样性阵列技术（DArT）标记在小黑麦中的应用。DArT 标记技术提供了高水平的多重性。通过从小黑麦品系中开发新标记，结合对先前在黑麦和小麦中开发的大量标记进行挖掘，从而开发出新的标记。使用三个基因分型阵列分析了 144 个小黑麦品系的集合。小麦和黑麦 DArT 标记的多态性水平分别为 8.6%至 23.8%。在多态性标记中，黑麦标记最多（3109 个），其次是小麦（2214 个）和小黑麦（719 个）。黑麦 DArT 标记的平均多态性信息含量值为 0.34，而小黑麦和小麦的标记分别为 0.37。黑麦、小黑麦、小麦和组合标记集衍生的相似性矩阵以及亲缘系数矩阵之间观察到高度相关性。聚类分析揭示了与农艺和系谱信息一致的供体之间的遗传关系。新开发的小黑麦 DArT 标记以及源自黑麦和小麦的标记提供了高质量的标记，可用于多样性分析，并可能在小黑麦的一系列分子育种和基因组学应用中得到利用。
