Zhu Z, Deng H, Fenderson B A, Nudelman E D, Tsui Z
Department of Biochemistry, Dalian Medical College, Liaoning, People's Republic of China.
J Reprod Fertil. 1990 Jan;88(1):71-9. doi: 10.1530/jrf.0.0880071.
The glycolipid composition of human myometrium and endometrium was examined at various stages of maturation and reproduction. The major neutral glycolipids of both myometrium and endometrium were identified by high-performance thin-layer chromatography as globo-series glycolipids, Gb3 and Gb4. The major acidic glycolipids (gangliosides) were identified similarly as GM3 and GD3, with lesser amounts of GM1, GD1a, and GT1b. During pregnancy, GD3 expression declined in both myometrium and endometrium, whereas GM3 expression increased. Reciprocal changes in GM3/GD3 expression were mirrored by appropriate changes in the glycosyltransferases required for their synthesis; alpha 2----3sialyltransferase activity increased approximately 3-fold during pregnancy, while alpha 2----8sialyltransferase activity declined to about 20%. The results focus attention on the glycolipids of uterine tissues, their regulation, and their possible role in reproduction and fertility.
在成熟和生殖的各个阶段，对人子宫肌层和子宫内膜的糖脂组成进行了检测。通过高效薄层色谱法鉴定出子宫肌层和子宫内膜的主要中性糖脂为球系列糖脂、Gb3和Gb4。主要酸性糖脂（神经节苷脂）同样被鉴定为GM3和GD3，GM1、GD1a和GT1b的含量较少。在怀孕期间，子宫肌层和子宫内膜中的GD3表达均下降，而GM3表达增加。GM3/GD3表达的相互变化反映在其合成所需糖基转移酶的相应变化中；α2----3唾液酸转移酶活性在怀孕期间增加约3倍，而α2----8唾液酸转移酶活性下降至约20%。这些结果将注意力集中在子宫组织的糖脂、它们的调节以及它们在生殖和生育中的可能作用上。
