Miyagi T, Tsuiki S
FEBS Lett. 1986 Oct 6;206(2):223-8. doi: 10.1016/0014-5793(86)80985-1.
Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of which had been considered to resist mammalian sialidases, were desialylated. The products of GM2 and GM1 hydrolysis were identified as asialo-GM2 and asialo-GM1, respectively, by thin-layer chromatography.
大鼠肝细胞膜从混合牛脑神经节苷脂中去除唾液酸的效率高于从唾液乳糖和类粘蛋白中去除唾液酸的效率，最适pH为4.5。当分别用[14C]唾液酸或[3H]鞘氨醇标记的单个神经节苷脂进行测试时，不仅GD1a和GM3，而且GM2和GM1（这两种神经节苷脂都曾被认为能抵抗哺乳动物唾液酸酶）都发生了去唾液酸化。通过薄层色谱法，GM2和GM1水解产物分别被鉴定为脱唾液酸GM2和脱唾液酸GM1。
