高迁移率族蛋白B1：一种用于估计死后间隔时间的新标志物。
HMGB1: A new marker for estimation of the postmortem interval.
作者信息
Kikuchi Kiyoshi, Kawahara Ko-Ichi, Biswas Kamal Krishna, Ito Takashi, Tancharoen Salunya, Shiomi Naoto, Koda Yoshiro, Matsuda Fumiyo, Morimoto Yoko, Oyama Yoko, Takenouchi Kazunori, Miura Naoki, Arimura Noboru, Nawa Yuko, Arimura Shinichiro, Jie Meng Xiao, Shrestha Binita, Iwata Masahiro, Mera Kentaro, Sameshima Hisayo, Ohno Yoshiko, Maenosono Ryuichi, Tajima Yutaka, Uchikado Hisaaki, Kuramoto Terukazu, Nakayama Kenji, Shigemori Minoru, Yoshida Yoshihiro, Hashiguchi Teruto, Maruyama Ikuro
机构信息
Division of Laboratory and Vascular Medicine, Field of Cardiovascular and Respiratory Disorders, Department of Advanced Therapeutics, and ; Department of Neurosurgery, Omuta City General Hospital, Omuta 836-8567;
出版信息
Exp Ther Med. 2010 Jan;1(1):109-111. doi: 10.3892/etm_00000019. Epub 2010 Jan 1.
Estimation of the postmortem interval (PMI) is one of the most important tasks in forensic medicine. Numerous methods have been proposed for the determination of the time since death by chemical means. High mobility group box-1 (HMGB1), a nonhistone DNA-binding protein is released by eukaryotic cells upon necrosis. Postmortem serum levels of HMGB1 of 90 male Wistar rats stored at 4, 14 and 24°C since death were measured by enzyme-linked immunosorbent assay. The serum HMGB1 level showed a time-dependent increase up to seven days at 4°C. At 14°C, the HMGB1 level peaked at day 3, decreased at day 4, and then plateaued. At 24°C, the HMGB1 level peaked at day 2, decreased at day 3, and then plateaued. Our findings suggest that HMGB1 is related to the PMI in rats.
死后间隔时间（PMI）的估计是法医学中最重要的任务之一。已经提出了许多通过化学方法确定死亡时间的方法。高迁移率族蛋白B1（HMGB1）是一种非组蛋白DNA结合蛋白，在细胞坏死时由真核细胞释放。采用酶联免疫吸附测定法检测了90只雄性Wistar大鼠死后分别在4℃、14℃和24℃保存时血清中HMGB1的水平。血清HMGB1水平在4℃下直至第7天呈时间依赖性升高。在14℃时，HMGB1水平在第3天达到峰值，第4天下降，然后趋于平稳。在24℃时，HMGB1水平在第2天达到峰值，第3天下降，然后趋于平稳。我们的研究结果表明，HMGB1与大鼠的PMI有关。
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