RIVM National Institute for Public Health and the Environment, Centre for Infectious Disease Control Netherlands, P,O, Box 1, 3720 BA, Bilthoven, the Netherlands.
Antimicrob Resist Infect Control. 2012 Nov 8;1(1):34. doi: 10.1186/2047-2994-1-34.
Studies frequently use nasal swabs to determine Staphylococcus aureus carriage. Self-sampling would be extremely useful in an outhospital research situation, but has not been studied in a healthy population. We studied the similarity of self-samples and investigator-samples in nares and pharynxes of healthy study subjects (hospital staff) in the Netherlands.
One hundred and five nursing personnel members were sampled 4 times in random order after viewing an instruction paper: 1) nasal self-sample, 2) pharyngeal self-sample, 3) nasal investigator-sample, and 4) pharyngeal investigator-sample.
For nasal samples, agreement is 93% with a kappa coefficient of 0.85 (95% CI 0.74-0.96), indicating excellent agreement, for pharyngeal samples agreement is 83% and the kappa coefficient is 0.60 (95% CI 0.43-0.76), indicating good agreement. In both sampling sites self-samples even detected more S. aureus than investigator-samples.
This means that self-samples are appropriate for detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.
研究经常使用鼻腔拭子来确定金黄色葡萄球菌的携带情况。自我采样在院外研究情况下非常有用，但尚未在健康人群中进行研究。我们研究了荷兰健康研究对象（医院工作人员）的鼻腔和咽部的自我样本和调查员样本之间的相似性。
105 名护理人员在观看指导手册后随机进行了 4 次采样：1）鼻腔自我采样，2）咽自我采样，3）鼻腔调查员采样，4）咽调查员采样。
对于鼻腔样本，一致性为 93%，kappa 系数为 0.85（95%CI 0.74-0.96），表明具有极好的一致性，对于咽样本，一致性为 83%，kappa 系数为 0.60（95%CI 0.43-0.76），表明具有良好的一致性。在两个采样部位，自我采样甚至比调查员采样检测到更多的金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌。
这意味着自我采样适用于金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的检测。
