Deitch E A, Lu Q, Xu D Z, Specian R D
Department of Surgery, LSU Medical Center, Shreveport 71130.
J Trauma. 1990 Mar;30(3):259-68. doi: 10.1097/00005373-199003000-00003.
Previously, we documented that humoral factors, especially complement products, contained in burn wound blister fluid (BF) modulate normal neutrophil (PMN) function and metabolism. The goal of the current study was to examine the effects of the local (BF) and systemic (burn serum or plasma) burn microenvironment on PMN activation as assessed by complement receptor expression and morphology. Induction of CR1 (C3b) and CR3 (iC3b) receptor expression of normal PMNs after incubation in medium, BF, or plasma (serum) from healthy volunteers or burned patients was measured using monoclonal antibodies and flow cytometry. BF (10% v/v, 37 degrees C) induced 25% more CR1 receptor expression than control or burn plasma (p less than 0.05), while the levels of CR3 expression of PMNs incubated in BF (10% or 80% v/v at 37 degrees C) was more than 300% that found when the PMNs were incubated in medium, burn, or patient plasma (p less than 0.05). The electron microscopic appearance of PMNs incubated in these fluids documented that degranulation was greater when cells were incubated in BF than medium, control, or patient plasma. These results indicate that PMN activation (CR1 and CR3 expression) is greater in cells exposed to the local (BF) than the systemic (plasma) humoral microenvironment shortly after thermal injury.
此前，我们记录到烧伤创面水疱液（BF）中所含的体液因子，尤其是补体产物，可调节正常中性粒细胞（PMN）的功能和代谢。本研究的目的是通过补体受体表达和形态学评估，研究局部（BF）和全身（烧伤血清或血浆）烧伤微环境对PMN激活的影响。使用单克隆抗体和流式细胞术测量正常PMN在培养基、BF或来自健康志愿者或烧伤患者的血浆（血清）中孵育后CR1（C3b）和CR3（iC3b）受体表达的诱导情况。BF（10% v/v，37℃）诱导的CR1受体表达比对照或烧伤血浆多25%（p<0.05），而在BF（10%或80% v/v，37℃）中孵育的PMN的CR3表达水平比在培养基、烧伤或患者血浆中孵育时高出300%以上（p<0.05）。在这些液体中孵育的PMN的电子显微镜外观表明，当细胞在BF中孵育时，脱颗粒比在培养基、对照或患者血浆中孵育时更明显。这些结果表明，热损伤后不久，暴露于局部（BF）体液微环境的细胞中PMN激活（CR1和CR3表达）比暴露于全身（血浆）体液微环境的细胞更强烈。
